Liquid chromatographic–mass spectrometric assay for simultaneous pyrimethamine and sulfadoxine determination in human plasma samples

We present a liquid chromatographic–mass spectrometric assay for the simultaneous determination of sulfadoxine and pyrimethamine in human plasma samples. Sample clean-up was achieved by adding acetonitrile for protein precipitation. Gradient elution in only 10 min resulted in high throughput capabil...

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Bibliographic Details
Published in:Journal of Chromatography A 2005-05, Vol.1076 (1), p.97-102
Main Authors: Sinnaeve, Bart A., Decaestecker, Tineke N., Risha, Peter G., Remon, Jean-Paul, Vervaet, Chris, Van Bocxlaer, Jan F.
Format: Article
Language:English
Subjects:
Analysis
Antimalarials - blood
Antimalarials - pharmacokinetics
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Humans
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Pyrimethamine
Pyrimethamine - blood
Pyrimethamine - pharmacokinetics
Sensitivity and Specificity
Sulfadoxine
Sulfadoxine - blood
Sulfadoxine - pharmacokinetics
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Summary:We present a liquid chromatographic–mass spectrometric assay for the simultaneous determination of sulfadoxine and pyrimethamine in human plasma samples. Sample clean-up was achieved by adding acetonitrile for protein precipitation. Gradient elution in only 10 min resulted in high throughput capability. Tandem mass spectrometric detection in multiple reaction monitoring was used for quantification. The developed analytical approach was successfully validated and was applied in the pharmacokinetic evaluation of the bioavailability between two sulfadoxine/pyrimethamine formulations available on the Eastern African market, using a cross-over design.
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.04.047