Molecular characterization of the kinetoplastid membrane protein-11 genes from the parasite Trypanosoma rangeli

Trypanosomatids are early divergent parasites which include several species of medical interest. Trypanosoma rangeli is not pathogenic for humans but shows a high immunological cross-reactivity with Trypanosoma cruzi, the causative agent of Chagas' disease that affects more than 17 million peop...

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Bibliographic Details
Published in:Parasitology 2005-06, Vol.130 (6), p.643-651
Main Authors: DIEZ, H., THOMAS, M. C., URUEÑA, C. P., SANTANDER, S. P., CUERVO, C. L., LÓPEZ, M. C., PUERTA, C. J.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosomes
Disease
Fundamental and applied biological sciences. Psychology
Gene Expression
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genes
Invertebrates
kinetoplastid membrane protein-11
Membrane Glycoproteins - chemistry
Membrane Glycoproteins - genetics
molecular characterization
Molecular Sequence Data
Parasites
Proteins
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Sequence Alignment
Trypanosoma - genetics
Trypanosoma - metabolism
Trypanosoma cruzi
Trypanosoma rangeli
Vector-borne diseases
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Summary:Trypanosomatids are early divergent parasites which include several species of medical interest. Trypanosoma rangeli is not pathogenic for humans but shows a high immunological cross-reactivity with Trypanosoma cruzi, the causative agent of Chagas' disease that affects more than 17 million people throughout the world. Recent studies have suggested that T. cruzi KMP-11 antigen could be a good candidate for the induction of immunoprotective cytotoxic responses against T. cruzi natural infection. In the present paper the genes coding for the T. rangeli kinetoplastid membrane protein-11 have been characterized. The results show that the locus encoding this protein is formed by 4 gene units measuring 550 nucleotides in length, organized in tandem, and located in different chromosomes in KP1(+) and KP1(−) strains. The gene units are transcribed as a single mRNA of 530 nucleotides in length. Alignment of the T. rangeli KMP-11 deduced amino acid sequence with the homologous KMP-11 protein from T. cruzi revealed an identity of 97%. Interestingly, the T and B cell epitopes of the T. cruzi KMP-11 protein are conserved in the T. rangeli KMP-11 amino acid sequence.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182004006936