Development of a High-Throughput Automated Analyzer Using Biochip Array Technology

Use of protein array technology over conventional assay methods has advantages that include simultaneous detection of multiple analytes, reduction in sample and reagent volumes, and high output of test results. The susceptibility of ligands to denaturation, however, has impeded production of a stabl...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2005-07, Vol.51 (7), p.1165-1176
Main Authors: FitzGerald, Stephen P, Lamont, John V, McConnell, Robert I, Benchikh, El O
Format: Article
Language:English
Subjects:
Aluminum
Analytical, structural and metabolic biochemistry
Antibodies
Arrays
Autoanalysis
Automation
Biochip products
Biological and medical sciences
Biomarkers
Biomarkers - analysis
Cytokines
Cytokines - analysis
Fabrication
Fundamental and applied biological sciences. Psychology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Laboratories
Ligands
Luminescent Measurements
Mass spectrometry
Medical sciences
Nanotechnology
Product development
Protein Array Analysis - methods
Proteins
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization
Spectrometry, X-Ray Emission
Surface chemistry
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Summary:Use of protein array technology over conventional assay methods has advantages that include simultaneous detection of multiple analytes, reduction in sample and reagent volumes, and high output of test results. The susceptibility of ligands to denaturation, however, has impeded production of a stable, reproducible biochip platform, limiting most array assays to manual or, at most, semiautomated processing techniques. Such limitations may be overcome by novel biochip fabrication procedures. After selection of a suitable biochip substrate, biochip surfaces were chemically modified and assessed to enable optimization of biochip fabrication procedures for different test panels. The assay procedure was then automated on a dedicated instrument, and assay performance was determined for a panel of cytokine markers. Assay results were then compared with a commercial method for measurement of cytokine markers. Secondary ion mass spectrometry and x-ray photoelectron spectroscopy demonstrated appropriate and reproducible modification of the biochip surface. Contact-angle studies also confirmed generation of hydrophobic surfaces that enabled containment of droplets for fabrication of discrete test regions. Automation of the biochip assays on a dedicated instrument produced excellent cytokine marker performance with intra- and interassay imprecision
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2005.049429