Clostridium perfringens: Insight into virulence evolution and population structure

• Published in: Anaerobe 2006-02, Vol.12 (1), p.23-43
• Main Authors: Sawires, Youhanna S., Songer, J. Glenn
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Toxins - classification; Bacterial Toxins - genetics; Biological Evolution; Clostridium perfringens; Clostridium perfringens - classification; Clostridium perfringens - genetics; Clostridium perfringens - pathogenicity; Clostridium perfringens - physiology; Evolution of virulence; Genetic Variation - genetics; Genotype; Humans; Minisatellite Repeats - genetics; MLVA; Phylogeny; Population structure; Positive selection; Selection, Genetic; Vertebrates - microbiology; Virulence
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2005.10.002

Clostridium perfringens is an important pathogen in veterinary and medical fields. Diseases caused by this organism are in many cases life threatening or fatal. At the same time, it is part of the ecological community of the intestinal tract of man and animals. Virulence in this species is not fully understood and it does seem that there is erratic distribution of the toxin/enzyme genes within C. perfringens population. We used the recently developed multiple-locus variable-number tandem repeat analysis (MLVA) scheme to investigate the evolution of virulence and population structure of this species. Analysis of the phylogenetic signal indicates that acquisition of the major toxin genes as well as other plasmidborne toxin genes is a recent evolutionary event and their maintenance is essentially a function of the selective advantage they confer in certain niches under different conditions. In addition, it indicates the ability of virulent strains to cause disease in different host species. More interestingly, there is evidence that certain normal flora strains are virulent when they gain access to a different host species. Analysis of the population structure indicates that recombination events are the major tool that shapes the population and this panmixia is interrupted by frequent clonal expansion that mostly corresponds to disease processes. The signature of positive selection was detected in alpha toxin gene, suggesting the possibility of adaptive alleles on the other chromosomally encoded determinants. Finally, C. perfringens proved to have a dynamic population and availability of more genome sequences and use of comparative proteomics and animal modeling would provide more insight into the virulence of this organism.