Positive electrospray ion trap multistage mass spectrometric fragmentation of synthetic analogs of saccharide part of lipopolysaccharides of Vibrio cholerae O:1

Oligosaccharides (mono- to hexamers) that mimic the terminal epitopes of O-antigens of Vibrio cholerae O:1, serotypes Ogawa and Inaba, have been studied by electrospray ion trap (ESI IT) mass spectrometry. Sodium or potassium-cationized adducts are characteristic ions under the conditions of ESI-MS...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2006-06, Vol.17 (6), p.749-756
Main Authors: KOVACIK, Vladimir, BEKESOVA, Slavka, TVAROSKA, Igor, KOVAC, Pavol
Format: Article
Language:English
Subjects:
Adducts
Analytical chemistry
Antigens
Antigens, Bacterial - chemistry
Bacteria
Bacteriology
Biological and medical sciences
Carbohydrate Conformation
Carbohydrates
Chemistry
Electrospraying
Exact sciences and technology
Fragmentation
Fragments
Fundamental and applied biological sciences. Psychology
Ions
Lipopolysaccharides - chemistry
Lipopolysaccharides - metabolism
Mass spectrometry
Microbiology
Molecular Structure
Multistage
Oligomers
Oligosaccharides
Organic chemistry
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Reactivity and mechanisms
Spectrometric and optical methods
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
Vibrio cholerae - chemistry
Vibrio cholerae - metabolism
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Summary:Oligosaccharides (mono- to hexamers) that mimic the terminal epitopes of O-antigens of Vibrio cholerae O:1, serotypes Ogawa and Inaba, have been studied by electrospray ion trap (ESI IT) mass spectrometry. Sodium or potassium-cationized adducts are characteristic ions under the conditions of ESI-MS analysis. The tentative pathways of fragmentation have been proven by multistage ion trap MS (MS(n), n = 1-3). The predominant pathway of fragmentation of the oligomers is the neutral loss of monosaccharide residue shortening the length of the oligosaccharide. In this way, conversion of the Ogawa to Inaba fragments takes place under the conditions of measurement. ESI MS/MS provided sufficient information about molecular mass, the number of saccharide residues, and the structure of saccharides, about the C (4)-amide of 3-deoxy-L-glycero-tetronic acid (DGT) of the compounds investigated, and allows to distinguish between Ogawa and Inaba serotypes.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2006.02.006