Meningiomas expressing and responding to cholecystokinin (CCK)

The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulatio...

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Bibliographic Details
Published in:Journal of neuro-oncology 2005-07, Vol.73 (3), p.199-204
Main Authors: OIKONOMOU, Eftychia, MACHADO, Ana L, BUCHFELDER, Michael, ADAMS, Eric F
Format: Article
Language:English
Subjects:
Adult
Base Sequence
Benzodiazepinones - pharmacology
Biological and medical sciences
Cell Proliferation - drug effects
Cells, Cultured
Cholecystokinin - genetics
Cholecystokinin - metabolism
Cholecystokinin - pharmacology
Female
Humans
Male
Medical sciences
Meningeal Neoplasms - genetics
Meningeal Neoplasms - metabolism
Meningioma - genetics
Meningioma - metabolism
Middle Aged
Molecular Sequence Data
Neurology
Neurosurgery
Phenylurea Compounds - pharmacology
Receptors, Cholecystokinin - antagonists & inhibitors
Receptors, Cholecystokinin - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Skull, brain, vascular surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Time Factors
Tumors of the nervous system. Phacomatoses
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Summary:The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism.
ISSN:0167-594X
1573-7373
DOI:10.1007/s11060-004-5177-2