Meningiomas expressing and responding to cholecystokinin (CCK)

• Published in: Journal of neuro-oncology 2005-07, Vol.73 (3), p.199-204
• Main Authors: OIKONOMOU, Eftychia, MACHADO, Ana L, BUCHFELDER, Michael, ADAMS, Eric F
• Format: Article
• Language: English
• Subjects: Adult; Base Sequence; Benzodiazepinones - pharmacology; Biological and medical sciences; Cell Proliferation - drug effects; Cells, Cultured; Cholecystokinin - genetics; Cholecystokinin - metabolism; Cholecystokinin - pharmacology; Female; Humans; Male; Medical sciences; Meningeal Neoplasms - genetics; Meningeal Neoplasms - metabolism; Meningioma - genetics; Meningioma - metabolism; Middle Aged; Molecular Sequence Data; Neurology; Neurosurgery; Phenylurea Compounds - pharmacology; Receptors, Cholecystokinin - antagonists & inhibitors; Receptors, Cholecystokinin - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Skull, brain, vascular surgery; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Time Factors; Tumors of the nervous system. Phacomatoses
• ISSN: 0167-594X; 1573-7373
• DOI: 10.1007/s11060-004-5177-2

The effect of cholecystokinin (CCK) on cultured human meningioma derived cells was investigated. Exposure of meningioma cells for 6-12 days to CCK-8s (2-200 nM) resulted in a dose dependent stimulation of cell growth to a maximum of 1.1-fold over basal controls. A time course study showed stimulation of cell growth at day 3 followed by increase throughout day 6. The stimulatory effect of CCK on meningioma cell growth was completely abolished by a CCK-B specific receptor antagonist, L-365,260. Reverse-transcription of meningioma-derived RNA into cDNA followed by amplification by the polymerase chain reaction using specific primers for CCK peptide and its CCK-A and/B receptor revealed 100% presence of CCK peptide and CCK-B receptors mRNA whereas CCK-A receptor was expressed in 66% of the meningiomas. These results provide evidence that human meningioma cells possess CCK peptide and its receptors the activation of which leads to increase of cell growth possibly via an autocrine/paracrine mechanism.