The Immunogenicity of Human Adipose‐Derived Cells: Temporal Changes In Vitro

Regenerative medical techniques will require an abundant source of human adult stem cells that can be readily available at the point of care. The ability to use unmatched allogeneic stem cells will help achieve this goal. Since adipose tissue represents an untapped reservoir of human cells, we have...

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Published in:Stem cells (Dayton, Ohio) Ohio), 2006-05, Vol.24 (5), p.1246-1253
Main Authors: McIntosh, Kevin, Zvonic, Sanjin, Garrett, Sara, Mitchell, James B., Floyd, Z. Elizabeth, Hammill, Lora, Kloster, Amy, Di Halvorsen, Yuan, Ting, Jenny P., Storms, Robert W., Goh, Brian, Kilroy, Gail, Wu, Xiying, Gimble, Jeffrey M.
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - immunology
Adipose Tissue - cytology
Adipose Tissue - immunology
Adipose‐derived stem cells
Antigens, CD - metabolism
Bone Marrow Cells - cytology
Cell Adhesion
Cell Separation
Cells, Cultured
Human
Humans
Immunogenic
Immunophenotyping
Immunosuppressive
Lymphocyte Activation
Mixed lymphocyte reaction
Multipotent
Regenerative medicine
Stromal Cells - cytology
T-Lymphocytes - immunology
Time Factors
Tissue engineering
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Summary:Regenerative medical techniques will require an abundant source of human adult stem cells that can be readily available at the point of care. The ability to use unmatched allogeneic stem cells will help achieve this goal. Since adipose tissue represents an untapped reservoir of human cells, we have compared the immunogenic properties of freshly isolated, collagenase‐digested human adipose tissue‐derived stromal vascular fraction cells (SVFs) relative to passaged, plastic‐adherent adipose‐derived stem cells (ASCs). Parallel studies have shown that adherence to plastic and subsequent expansion of human adipose‐derived cells selects for a relatively homogeneous cell population based on immunophenotype. Consistent with these findings, the presence of hematopoietic‐associated markers (CD11a, CD14, CD45, CD86, and histocompatible locus antigen‐DR [HLA‐DR]) detected on the heterogeneous SVF cell population decreased upon subsequent passage of the ASCs. In mixed lymphocyte reactions (MLRs), SVFs, and early passage ASCs stimulated proliferation by allogeneic responder T cells. In contrast, the ASCs beyond passage P1 failed to elicit a response from T cells. Indeed, late passage ASCs actually suppressed the MLR response. Although these results support the feasibility of allogeneic human ASC transplantation, confirmatory in vivo animal studies will be required.
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.2005-0235