Reproducibility of MDM2 and CDK4 staining in soft tissue tumors

MDM2 and CDK4 immunostaining can be useful adjuncts in diagnosing liposarcoma among soft tissue neoplasms. We examined the reproducibility of MDM2 and CDK4 staining between 2 laboratories and between tissue microarrays and whole tissue sections. Sixty-two soft tissue tumors were immunostained at the...

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Bibliographic Details
Published in:American journal of clinical pathology 2006-05, Vol.125 (5), p.693-697
Main Authors: BUI NGUYEN BINH, Matthieu, SASTRE GARAU, Xavier, GUILLOU, Louis, AURIAS, Alain, COINDRE, Jean-Michel
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomarkers, Tumor - analysis
Humans
Immunoenzyme Techniques
Investigative techniques, diagnostic techniques (general aspects)
Keratins - analysis
Medical sciences
Observer Variation
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Proto-Oncogene Proteins c-mdm2 - analysis
Reproducibility of Results
Retrospective Studies
Sarcoma - chemistry
Sarcoma - pathology
Soft Tissue Neoplasms - chemistry
Soft Tissue Neoplasms - pathology
Tissue Array Analysis
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Summary:MDM2 and CDK4 immunostaining can be useful adjuncts in diagnosing liposarcoma among soft tissue neoplasms. We examined the reproducibility of MDM2 and CDK4 staining between 2 laboratories and between tissue microarrays and whole tissue sections. Sixty-two soft tissue tumors were immunostained at the Bergonié Institute, Bordeaux, France, and the Curie Institute, Paris, France. We also examined 203 soft tissue neoplasms on standard tissue sections and tissue microarrays. There was high concordance of results obtained from the 2 laboratories (with 2 different pathologists) for MDM2 (kappa, 0.93) and CDK4 (kappa, 0.8) staining. There also was excellent concordance between results on tissue microarray and on whole tissue sections for MDM2 (kappa, 0.80) and CDK4 (kappa, 0.93). Immunostaining for MDM2 and CDK4 is a reproducible technique that may be exported to different laboratories for routine use. Tissue microarray is indicated for studying large series.
ISSN:0002-9173
1943-7722
DOI:10.1309/VMBP67QUNN6Q3J0E