A functional polymorphism in MMP-9 is associated with childhood atopic asthma

Although MMP-9 has been suggested to be important in inflammation and in the connective tissue remodeling associated with asthma, the genetic influences of the polymorphisms of MMP-9 are unclear. To examine whether polymorphisms in MMP-9 are associated with childhood atopic asthma, we identified a t...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-05, Vol.344 (1), p.300-307
Main Authors: Nakashima, Kazuko, Hirota, Tomomitsu, Obara, Kazuhiko, Shimizu, Makiko, Doi, Satoru, Fujita, Kimie, Shirakawa, Taro, Enomoto, Tadao, Yoshihara, Shigemi, Ebisawa, Motohiro, Matsumoto, Kenji, Saito, Hirohisa, Suzuki, Yoichi, Nakamura, Yusuke, Tamari, Mayumi
Format: Article
Language:English
Subjects:
5' Flanking Region
Adolescent
Association study
Asthma - genetics
Bronchial asthma
Child
Child, Preschool
Female
Gene Expression
Gene Frequency
Genetic Predisposition to Disease
Haplotype
Haplotypes
Humans
Linkage disequilibrium
Luciferases - analysis
Luciferases - genetics
Lung - cytology
Lung - metabolism
Male
Matrix Metalloproteinase 9 - genetics
MMP-9
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Polymorphisms
Promoter Regions, Genetic
Reporter gene assay
Respiratory Mucosa - metabolism
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although MMP-9 has been suggested to be important in inflammation and in the connective tissue remodeling associated with asthma, the genetic influences of the polymorphisms of MMP-9 are unclear. To examine whether polymorphisms in MMP-9 are associated with childhood atopic asthma, we identified a total of 17 polymorphisms and conducted an association study with asthma ( n = 290) and controls ( n = 638). 2127G>T and 5546G>A (R668Q) were significantly associated with the risk of childhood atopic asthma ( p = 0.0032 and 0.0016, respectively). In haplotype analysis, we also found a positive association with a haplotype ( p = 0.0053). MMP-9 was expressed in cultured human bronchial epithelial cells, and the mRNA expression level was upregulated by dsRNA. Furthermore, the promoter SNP −1590C>T, in strong linkage disequilibrium with 2127G>T, enhanced the transcriptional level of MMP-9. Thus, the MMP-9 gene might be involved in the development of asthma through functional genetic polymorphisms.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.03.102