Successful 40-hour preservation of the canine small intestine with the cavitary 2-layer method with glutamine supplementation

• Published in: Surgery 2006-05, Vol.139 (5), p.646-652
• Main Authors: Li, Shiri, Suzuki, Yasuyuki, Fujino, Yasuhiro, Kakinoki, Keiichiro, Yoshikawa, Takuro, Tanaka, Tomohiro, Goto, Naohiro, Tanioka, Yasuki, Sakai, Tetsuya, Kuroda, Yoshikazu
• Format: Article
• Language: English
• Subjects: Adenosine Diphosphate - metabolism; Amino Acids - metabolism; Animals; Biological and medical sciences; Blood Glucose - metabolism; Dogs; Female; General aspects; Glutamine - pharmacology; Intestinal Absorption; Intestinal Mucosa - drug effects; Intestinal Mucosa - physiology; Intestine, Small - drug effects; Intestine, Small - physiology; Intestine, Small - transplantation; Kinetics; Male; Maltose - metabolism; Medical sciences; Organ Preservation - methods
• ISSN: 0039-6060; 1532-7361
• DOI: 10.1016/j.surg.2005.10.005

We recently reported that the cavitary 2-layer method (cTLM) allowed stable 24-hour preservation of canine intestine. The aim of this study was to examine the possibility of the 40-hour preservation by cTLM that is supplemented with glutamine. Canine jejunal segments (40 cm) were allotransplanted heterotopically without storage (group 1), after 40-hour cold storage with University of Wisconsin solution (group 2), cTLM (group 3), UW with 2% glutamine (group 4), or cTLM with 2% glutamine (group 5). Mucosal glutamine, histidine, tryptophan, glutathione, and adenosine triphosphate concentrations were determined immediately after preservation. At day 7 after the transplantation, maltose absorption test and histopathologic analysis were performed. Mucosal glutamine concentrations increased significantly with glutamine supplementation during preservation in groups 4 and 5 ( P < .01). Mucosal adenosine triphosphate levels in cTLM groups (groups 3 and 5) were similar to those in group 1; group 4 showed very low levels after preservation, despite glutamine supplementation. Mucosal glutathione did not differ among groups 2, 3, 4, and 5 and were significantly lower than in group 1. In the absorption test, serum glucose curves showed a peak level by 30 minutes in groups 1 and 5; delayed peaks were seen in groups 2, 3, and 4 compared with group 1. The villous heights were 879, 555, 685, 688, and 773 μm in groups 1, 2, 3, 4, and 5, respectively (group 1 vs group 2, 3, and 4: P < .05). cTLM that was supplemented with glutamine extended a safe preservation period up to 40 hours in canine segmental small intestinal transplantation, although this study failed to elucidate a precise mechanism of the glutamine beneficial effect on the graft mucosa.