Molecular cloning and analysis of gonadal expression of Foxl2 in the medaka, Oryzias latipes

Foxl2 is a member of the winged helix/forkhead family of transcription factors and is known to be involved in ovarian development in some vertebrates. To address the role of Foxl2 in ovarian differentiation in medaka, we isolated Foxl2 cDNA and analyzed its expression patterns during sex differentia...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-05, Vol.344 (1), p.353-361
Main Authors: Nakamoto, Masatoshi, Matsuda, Masaru, Wang, De-Shou, Nagahama, Yoshitaka, Shibata, Naoki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aromatase
Aromatase - genetics
Cell Differentiation - genetics
Cloning, Molecular
Estrogens - pharmacology
Female
Fish Proteins - genetics
Fish Proteins - metabolism
Folliculogenesis
Forkhead Transcription Factors - genetics
Forkhead Transcription Factors - metabolism
Foxl2
Freshwater
Granulosa cell
Granulosa Cells - cytology
Granulosa Cells - metabolism
Hermaphroditic Organisms
Male
Medaka
Molecular Sequence Data
Oryzias - genetics
Oryzias - growth & development
Oryzias latipes
Ovarian development
Ovary - cytology
Ovary - growth & development
Ovary - metabolism
RNA, Messenger - analysis
RNA, Messenger - metabolism
Sequence Alignment
Sex Determination Processes
Sex differentiation
Sex Differentiation - genetics
Teleost
Testis - cytology
Testis - drug effects
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Foxl2 is a member of the winged helix/forkhead family of transcription factors and is known to be involved in ovarian development in some vertebrates. To address the role of Foxl2 in ovarian differentiation in medaka, we isolated Foxl2 cDNA and analyzed its expression patterns during sex differentiation. Expression of Foxl2 started in somatic cells surrounding germ cells in XX gonads, just after initiation of ovarian differentiation, and was maintained in granulosa cells throughout ovarian development. In the adult ovary, Foxl2 was expressed in previtellogenic and vitellogenic follicles, but expression ceased in postvitellogenic follicles. In contrast, Foxl2 mRNA could not be detected in testes. In addition, Foxl2 and aromatase mRNAs were co-localized in some somatic cells located on the ventral side of developing XX gonads. Our results suggested that Foxl2 was not involved in ovarian determination, but was involved in differentiation of granulosa cells in medaka.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.03.137