BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

• Published in: The Journal of parasitology 2005-04, Vol.91 (2), p.284-292
• Main Authors: Hassan, S. M T, Maache, M, Díaz de la Guardia, R, Córdova, O. M, Gil García, J. R, Galiana, M, Acuña Castroviejo, D, Martins, M, Osuna, Antonio
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Bile acids; Biochemistry; Biological and medical sciences; Carrier Proteins - analysis; Carrier Proteins - chemistry; Carrier Proteins - isolation & purification; Carrier Proteins - metabolism; Chromatography; Chromatography, Affinity; Cytoplasm; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Ethanol; Fatty acid-binding protein; Fatty Acid-Binding Proteins; Fatty acids; Fluorescence; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; Gels; General aspects; General aspects and techniques. Study of several systematic groups. Models; Giardia; Giardia lamblia; Giardia lamblia - chemistry; Giardia lamblia - metabolism; Giardia lamblia - ultrastructure; Immunohistochemistry; Immunoprecipitation; Invertebrates; Isoelectric Focusing; Isoelectric Point; Ligands; Lipids; Microscopy, Immunoelectron; Molecular Weight; MOLECULAR-CELL BIOLOGY; Oleic acid; Parasitology; Polyclonal antibodies; Proteins; Protozoan Proteins - analysis; Protozoan Proteins - chemistry; Protozoan Proteins - isolation & purification; Protozoan Proteins - metabolism; Scatchard analysis; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Testosterone; Tocopherol; Trophozoites; Vacuoles; Vitamin E
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-3352

We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and α-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles.