BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA

We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when...

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Published in:The Journal of parasitology 2005-04, Vol.91 (2), p.284-292
Main Authors: Hassan, S. M T, Maache, M, Díaz de la Guardia, R, Córdova, O. M, Gil García, J. R, Galiana, M, Acuña Castroviejo, D, Martins, M, Osuna, Antonio
Format: Article
Language:English
Subjects:
Animals
Antibodies
Bile acids
Biochemistry
Biological and medical sciences
Carrier Proteins - analysis
Carrier Proteins - chemistry
Carrier Proteins - isolation & purification
Carrier Proteins - metabolism
Chromatography
Chromatography, Affinity
Cytoplasm
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Ethanol
Fatty acid-binding protein
Fatty Acid-Binding Proteins
Fatty acids
Fluorescence
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Gels
General aspects
General aspects and techniques. Study of several systematic groups. Models
Giardia
Giardia lamblia
Giardia lamblia - chemistry
Giardia lamblia - metabolism
Giardia lamblia - ultrastructure
Immunohistochemistry
Immunoprecipitation
Invertebrates
Isoelectric Focusing
Isoelectric Point
Ligands
Lipids
Microscopy, Immunoelectron
Molecular Weight
MOLECULAR-CELL BIOLOGY
Oleic acid
Parasitology
Polyclonal antibodies
Proteins
Protozoan Proteins - analysis
Protozoan Proteins - chemistry
Protozoan Proteins - isolation & purification
Protozoan Proteins - metabolism
Scatchard analysis
Spectrometry, Fluorescence
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Testosterone
Tocopherol
Trophozoites
Vacuoles
Vitamin E
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cited_by cdi_FETCH-LOGICAL-b419t-8c3aed2c02e4b2f12b42ade7ced2a93ac9f3e4c4c1e9f875b40005c01f4374113
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container_issue 2
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container_title The Journal of parasitology
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creator Hassan, S. M T
Maache, M
Díaz de la Guardia, R
Córdova, O. M
Gil García, J. R
Galiana, M
Acuña Castroviejo, D
Martins, M
Osuna, Antonio
description We describe here a fatty acid–binding protein (FABP) isolated and purified from the parasitic protozoon Giardia lamblia. The protein has a molecular mass of 8 kDa and an isoelectric point of 4.96. A Scatchard analysis of the data at equilibrium revealed a dissociation constant of 3.12 × 10−8 M when the labeled oleic acid was displaced by a 10-fold greater concentration of unlabeled oleic acid. Testosterone, sodium desoxycholate, taurocholate, metronidazol, and α-tocopherol, together with butyric, arachidonic, palmitic, retinoic, and glycocholic acids, were also bound to the protein. Assays with polyclonal antibodies revealed that the protein is located in the ventral disk and also appears in the dorsal membrane, the cytoplasm, and in the vicinity of the lipid vacuoles.
doi_str_mv 10.1645/GE-3352
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identifier ISSN: 0022-3395
ispartof The Journal of parasitology, 2005-04, Vol.91 (2), p.284-292
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subjects Animals
Antibodies
Bile acids
Biochemistry
Biological and medical sciences
Carrier Proteins - analysis
Carrier Proteins - chemistry
Carrier Proteins - isolation & purification
Carrier Proteins - metabolism
Chromatography
Chromatography, Affinity
Cytoplasm
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Ethanol
Fatty acid-binding protein
Fatty Acid-Binding Proteins
Fatty acids
Fluorescence
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Gels
General aspects
General aspects and techniques. Study of several systematic groups. Models
Giardia
Giardia lamblia
Giardia lamblia - chemistry
Giardia lamblia - metabolism
Giardia lamblia - ultrastructure
Immunohistochemistry
Immunoprecipitation
Invertebrates
Isoelectric Focusing
Isoelectric Point
Ligands
Lipids
Microscopy, Immunoelectron
Molecular Weight
MOLECULAR-CELL BIOLOGY
Oleic acid
Parasitology
Polyclonal antibodies
Proteins
Protozoan Proteins - analysis
Protozoan Proteins - chemistry
Protozoan Proteins - isolation & purification
Protozoan Proteins - metabolism
Scatchard analysis
Spectrometry, Fluorescence
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Testosterone
Tocopherol
Trophozoites
Vacuoles
Vitamin E
title BINDING PROPERTIES AND IMMUNOLOCALIZATION OF A FATTY ACID–BINDING PROTEIN IN GIARDIA LAMBLIA
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