Isolation and characterization of a polymorphic stigma-specific class III peroxidase gene from Senecio squalidus L. (Asteraceae)

• Published in: Plant molecular biology 2005-03, Vol.57 (5), p.659-677
• Main Authors: McInnis, S.M, Costa, L.M, Gutierrez-Marcos, J.F, Henderson, C.A, Hiscock, S.J
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; amino acid sequences; Amino acids; Asteraceae; Base Sequence; Blotting, Northern; Blotting, Southern; cell walls; Cloning, Molecular; complementary DNA; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; DNA, Plant - chemistry; DNA, Plant - isolation & purification; enzyme activity; Enzymes; exons; Fertility - genetics; Flowers; Flowers - enzymology; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; genes; Genotype; In Situ Hybridization; introns; Isoelectric Focusing; Isoenzymes - chemistry; Isoenzymes - genetics; Isoenzymes - metabolism; messenger RNA; Molecular Sequence Data; nucleotide sequences; Pathogens; peroxidase; Peroxidase - chemistry; Peroxidase - genetics; Peroxidase - metabolism; Phylogeny; Plant species; Pollen; Polymorphism, Genetic; protein transport; Proteins; RNA, Plant - genetics; RNA, Plant - metabolism; Senecio - enzymology; Senecio - genetics; Senecio squalidus; Sequence Analysis, DNA; Sequence Analysis, Protein; Sequence Homology, Amino Acid; signal peptide; SSWP gene; stigma
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-005-1426-9

A novel stigma-specific class III peroxidase gene, SSP (Stigma-Specific Peroxidase), has been isolated from the self-incompatible daisy Senecio squalidus L. (Asteraceae). Expression of SSP in flower buds is developmentally regulated, with maximal levels of expression coinciding with anthesis, when stigmas are most receptive to pollen and when self-incompatibility is fully developed. In situ hybridization revealed SSP expression to be localized exclusively to the specialized secretory epidermal cells (papillae) of the stigma, which receive and discriminate pollen. SSP is therefore the first tissue-specific and cell-specific peroxidase gene identified in a plant. SSP belongs to a distinct clade of class III plant peroxidases that possess two introns, instead of the more normal situation of three conserved introns. The deduced amino acid sequence of SSP revealed a 27 amino acid signal peptide, suggesting that the SSP protein is secreted to the cell wall of the stigmatic papillae. In-gel peroxidase activity assays showed that SSP has relatively low peroxidase activity compared to other, as yet uncharacterized, peroxidases present in stigmatic extracts. Six SSP alleles have been cloned from different lines of S. squalidus carrying a range of self-incompatibility (S)-alleles but there was no consistent association between the presence of a particular SSP allele and S-genotype indicating that SSP is not the female determinant of SSI in S. squalidus. Nevertheless, the precise expression of SSP in stigmatic papillae suggests that it may have a more general function in pollen-stigma interactions, or alternatively in protection of stigmas from pathogen attack. Extensive database screens have identified homologues of SSP in other plant species, but available expression data for these genes indicates that none are flower-specific, suggesting that SSP represents a new functional type of class III peroxidase specific to the stigma. We discuss the possible function(s) of S. squalidus SSP in pollen-stigma interactions and in protection of stigmas from pathogen attack.