Extracellular calcium is required for the maintenance of plasma membrane integrity in nucleated cells

In contrast to rat and human erythrocytes, nucleated erythrocytes from two fish species ( Cyprinus carpio and Salmo trutta) underwent almost complete haemolysis in 20 min of EDTA addition. Using Ca 2+/Mg 2+ EGTA-citrate buffer, we observed that half-maximal haemolysis of fish erythrocytes occurs at...

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Bibliographic Details
Published in:Cell calcium (Edinburgh) 2005-07, Vol.38 (1), p.53-57
Main Authors: Orlov, Sergei N., Aksentsev, Sergei L., Kotelevtsev, Sergei V.
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Cell Membrane - metabolism
Cell Nucleus - metabolism
Cyprinus carpio
Egtazic Acid - analogs & derivatives
Egtazic Acid - pharmacology
Erythrocytes - cytology
Erythrocytes - drug effects
Erythrocytes - metabolism
Extracellular Ca
Fish erythrocytes
Fishes
Freshwater
HeLa Cells
Hemoglobins - metabolism
Hemolysis - drug effects
Humans
Integrity
L-Lactate Dehydrogenase - metabolism
Magnesium - pharmacology
Male
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Permeability for Na
Plasma membrane
Rats
Rats, Wistar
Salmo trutta
Smooth muscle
Sodium - metabolism
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Summary:In contrast to rat and human erythrocytes, nucleated erythrocytes from two fish species ( Cyprinus carpio and Salmo trutta) underwent almost complete haemolysis in 20 min of EDTA addition. Using Ca 2+/Mg 2+ EGTA-citrate buffer, we observed that half-maximal haemolysis of fish erythrocytes occurs at [Ca 2+] o ∼10 μM independently of extracellular Mg 2+ concentration. Attenuation of [Ca 2+] o with EGTA also decreased stability of the plasma membrane of vascular smooth muscle cells (VSMC) and HeLa cells, indicated by a three- to five-fold elevation of lactate dehydrogenase release and passive permeability of plasma membrane for Na +. In VSMC, EGTA lowered [Ca 2+] i by ∼20%. This effect was absent in VSMC-loaded with the intracellular Ca 2+ chelator BAPTA. In contrast to EGTA, BAPTA did not affect haemoglobin release from fish erythrocytes and passive permeability for Na + in VSMC. Viewed collectively, our data show that in nucleated cells, extracellular Ca 2+ plays a crucial role in the maintenance of plasma membrane integrity.
ISSN:0143-4160
1532-1991
DOI:10.1016/j.ceca.2005.03.006