Effects of mechanical wounding on Carica papaya cysteine endopeptidases accumulation and activity

The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits...

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Bibliographic Details
Published in:International journal of biological macromolecules 2006-05, Vol.38 (3), p.216-224
Main Authors: Azarkan, Mohamed, Dibiani, Rachid, Baulard, Céline, Baeyens-Volant, Danielle
Format: Article
Language:English
Subjects:
Carica - metabolism
Cations
Chitinases - chemistry
Chromatography
Chromatography, Ion Exchange
Endopeptidases - chemistry
Fruit
Latex - metabolism
Macromolecular Substances - chemistry
Papain
Plant defense
Pro-enzyme
Spectrophotometry
Stress, Mechanical
Wounding
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Summary:The mechanical wounding impact on the Carica papaya latex protein pattern was investigated by analyzing three latexes. A first one commercially available, a second harvested from unripe but fully grown fruits, both obtained from regularly tapped fruits. A third one was collected from similar fruits but wounded for the first time. The results demonstrated both quantitative and qualitative changes in the protein content and in the enzymatic activity. Repeated wounding results in either, accumulation or activation (or both of them) of papain, chymopapain and caricain. Furthermore, new cysteine protease activity was found to transiently accumulate in the latex collected from newly wounded fruits. The possible implication of this enzymatic material in the papaya cysteine endopeptidases pro-forms activation is discussed.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2006.02.021