Neutrophil gelatinase-associated lipocalin, a siderophore-binding eukaryotic protein

NGAL (neutrophil gelatinase-associated lipocalin) also known as lcn2 or siderochalin is constitutively expressed in myelocytes and stored in specific granules of neutrophils. It is highly induced in a variety of epithelial cells during inflammation. Analysis of the crystal structure of NGAL expresse...

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Bibliographic Details
Published in:Biometals 2006-04, Vol.19 (2), p.211-215
Main Authors: Borregaard, Niels, Cowland, Jack B
Format: Article
Language:English
Subjects:
Acute-Phase Proteins - metabolism
Animals
E coli
Epithelial Cells - metabolism
Humans
Iron
Iron - metabolism
Lipocalin-2
Lipocalins
Models, Biological
Neutrophils - metabolism
Oncogene Proteins - metabolism
Protein Binding
Proteins
Proto-Oncogene Proteins - metabolism
Rodents
Siderophores - metabolism
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Summary:NGAL (neutrophil gelatinase-associated lipocalin) also known as lcn2 or siderochalin is constitutively expressed in myelocytes and stored in specific granules of neutrophils. It is highly induced in a variety of epithelial cells during inflammation. Analysis of the crystal structure of NGAL expressed in E.coli showed that NGAL has the ability to bind catecholate type siderophores and in this way prevent bacteria from acquisition of siderophore-bound iron. NGAL (or 24p3 as the highly homologous murine orthologue is named) knock out mice have a profound defect in defense against E.coli after intraperitoneal injection. This defect can be mimicked in wild-type mice by providing siderophore iron, which cannot be sequestered by NGAL, testifying to the specific role of NGAL as a siderophore binding protein in innate immunity. Megalin, a scavenger receptor functions as a receptor for NGAL and mediates uptake into endosomes, but other NGAL receptors are likely to exist.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-005-3251-7