Chronic hypoxic decreases in soluble guanylate cyclase protein and enzyme activity are age dependent in fetal and adult ovine carotid arteries

• Published in: Journal of applied physiology (1985) 2006-06, Vol.100 (6), p.1857-1866
• Main Authors: Williams, James M, White, Charles R, Chang, Melody M, Injeti, Elisha R, Zhang, Lubo, Pearce, William J
• Format: Article
• Language: English
• Subjects: 1-Methyl-3-isobutylxanthine - pharmacology; Age differences; Aging - physiology; Animals; Biological and medical sciences; Carotid Arteries - embryology; Carotid Arteries - enzymology; Circulatory system; Cyclic GMP - analogs & derivatives; Cyclic GMP - analysis; Cyclic GMP - pharmacology; Cyclic GMP - physiology; Cyclic GMP-Dependent Protein Kinases - analysis; Cyclic GMP-Dependent Protein Kinases - physiology; Dose-Response Relationship, Drug; Enzyme Activation; Enzymes; Female; Fundamental and applied biological sciences. Psychology; Guanylate Cyclase - metabolism; Hypoxia - physiopathology; Nitric oxide; Nitric Oxide - physiology; Phosphodiesterase Inhibitors - pharmacology; Pregnancy; Pregnancy, Animal - physiology; RNA, Messenger - analysis; Sheep; Sheep - physiology; Thionucleotides - pharmacology; Veins & arteries
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00662.2005

Departments of Physiology, Pharmacology, and Biochemistry, Center for Perinatal Biology, Loma Linda University School of Medicine, Loma Linda, California Submitted 3 June 2005 ; accepted in final form 6 February 2006 The present study tests the hypothesis that chronic hypoxia enhances reactivity to nitric oxide (NO) through age-dependent increases in soluble guanylate cyclase (sGC) and protein kinase G (PKG) activity. In term fetal and adult ovine carotids, chronic hypoxia had no significant effect on mRNA levels for the 1 -subunit of sGC, but depressed sGC abundance by 16% in fetal and 50% in adult arteries, through possible depression of rates of mRNA translation (15% in fetal and 50% in adult) and/or increased protein turnover. Chronic hypoxia also depressed the catalytic activity of sGC, but only in fetal arteries (63%). Total sGC activity was reduced by chronic hypoxia in both fetal (69%) and adult (37%) carotid homogenates, but this effect was not observed in intact arteries when sGC activity was measured by timed accumulation of cGMP. In intact arteries treated with 300 µM 3-isobutyl-1-methylxanthine (IBMX), chronic hypoxia dramatically enhanced sGC activity in fetal (186%) but not adult (89%) arteries. This latter observation suggests that homogenization either removed an sGC activator, released an sGC inhibitor, or altered the phosphorylation state of the enzyme, resulting in reduced activity. In the absence of IBMX, chronic hypoxia had no significant effect on rates of cGMP accumulation. Chronic hypoxia also depressed the ability of the cGMP analog, 8-( p -chlorophenylthio)-cGMP, to promote vasorelaxation in both fetal (8%) and adult (12%) arteries. Together, these results emphasize the fact that intact and homogenized artery studies of sGC activity do not always yield equivalent results. The results further suggest that enhancement of reactivity to NO by chronic hypoxia must occur upstream of PKG and can only be possible if changes in cGMP occurred in functional compartments that afforded either temporal or chemical protection to the actions of phosphodiesterase. The range and age dependence of hypoxic effects observed also suggest that some responses to hypoxia must be compensatory and homeostatic, with reactivity to NO as the primary regulated variable. cyclic guanosine 3',5'-cyclic monophosphate; protein kinase G; vascular maturation Address for reprint requests and other correspondence: W. J. Pearce, Center for Perinatal Biology, Loma Linda