Cell proliferation and apoptosis in the anterior intestine of an amphibious, euryhaline mudskipper (Periophthalmus modestus)

In order to replace the diffusive loss of water to the surrounding environment, seawater (SW)-acclimated euryhaline fishes have gastrointestinal tracts with higher ion/water flux in concert with greater permeability, and contrast that to freshwater (FW)-acclimated fish. To understand the cellular ba...

Full description

Saved in:
Bibliographic Details
Published in:Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Biochemical, systemic, and environmental physiology, 2006-06, Vol.176 (5), p.463-468
Main Authors: Takahashi, H, Sakamoto, T, Narita, K
Format: Article
Language:English
Subjects:
Acclimatization
Acclimatization - physiology
Animals
Apoptosis
Apoptosis - physiology
Brackish
Cell Differentiation - physiology
Cell Membrane Permeability - physiology
Cell Proliferation
Epithelium - metabolism
Female
Fresh Water
Freshwater fish
Gastrointestinal tract
Intestines - cytology
Intestines - physiology
Male
Marine
Perciformes - anatomy & histology
Perciformes - physiology
Periophthalmus modestus
Permeability
Proteins
Seawater
Water-Electrolyte Balance - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to replace the diffusive loss of water to the surrounding environment, seawater (SW)-acclimated euryhaline fishes have gastrointestinal tracts with higher ion/water flux in concert with greater permeability, and contrast that to freshwater (FW)-acclimated fish. To understand the cellular basis for these differences, we examined cell proliferation and apoptosis in the anterior intestine of mudskipper transferred from one-third SW to FW or to SW for 1 and 7 days, and those kept out of water for 1 day. The intestinal apoptosis (indicated by DNA laddering) increased during seawater acclimation. TUNEL staining detected numerous apoptotic cells over the epithelium of SW-acclimated fish. Cell proliferation ([3H]thymidine incorporation) in the FW fish was greater than those in SW 7 days after transfer. Labeling with a Proliferating cell nuclear antigen (PCNA) antibody indicated that proliferating cells were greater in number and randomly distributed in the epithelium of FW fish, whereas in SW fish they were almost entirely in the troughs of the intestinal folds. There were no changes in cell turnover in fish kept out of water. During acclimation to different salinities, modification of the cell turnover and abundance may play an important role in regulating the permeability (and transport capacity) of the gastrointestinal tract of fish.
ISSN:0174-1578
1432-136X
DOI:10.1007/s00360-006-0067-x