tRNA Actively Shuttles between the Nucleus and Cytosol in Yeast

Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription....

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2005-07, Vol.309 (5731), p.140-142
Main Authors: Takano, Akira, Endo, Toshiya, Yoshihisa, Tohru
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Adenosine Triphosphate - metabolism
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cell growth
Cell nucleus
Cell Nucleus - genetics
Cell Nucleus - metabolism
Cell physiology
Cells
Cytosol
Cytosol - metabolism
Energy
Fundamental and applied biological sciences. Psychology
Heterokaryon
Imports
Introns
Membrane and intracellular transports
Molecular and cellular biology
Mutation
Nucleic acids
ran GTP-Binding Protein - metabolism
RNA Precursors - metabolism
RNA, Fungal - metabolism
Rna, ribonucleoproteins
RNA, Transfer - genetics
RNA, Transfer - metabolism
RNA, Transfer, Amino Acid-Specific - metabolism
RNA, Transfer, Amino Acyl - metabolism
RNA, Transfer, Ile - metabolism
RNA, Transfer, Pro - metabolism
RNA, Transfer, Tyr - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Transcription, Genetic
Transcripts (Written Records)
Transfer RNA
Yeast
Yeasts
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Description
Summary:Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1113346