Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)

It has been reported that the copper (Cu) content of hepatocytes increases in chronic liver diseases and small hepatocellular carcinoma (HCC). In cells, Cu exists mainly as Cu-metallothionein (MT) or Cu, zinc (Zn)-superoxide dismutase (SOD). In this study, we investigated the biochemical state of Cu...

Full description

Saved in:
Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2005, Vol.28(7), pp.1137-1141
Main Authors: Kubo, Satoshi, Fukuda, Hiroyuki, Ebara, Masaaki, Ikota, Nobuo, Saisho, Hiromitsu, Nakagawa, Hidehiko, Ozawa, Toshihiko, Yukawa, Masae, Kato, Kazuki, Satoh, Tsunenobu, Watayo, Takaho, Sakurai, Hiromu
Format: Article
Language:English
Subjects:
Aged
Carcinoma, Hepatocellular - enzymology
Carcinoma, Hepatocellular - metabolism
Chromatography, High Pressure Liquid - methods
Chronic Disease
Copper - metabolism
Female
hepatocellular carcinoma
high-performance liquid chromatography
Humans
Liver Neoplasms - enzymology
Liver Neoplasms - metabolism
Male
metallothionein
Metallothionein - chemistry
Middle Aged
particle-induced X-ray emission
superoxide dismutase
Superoxide Dismutase - metabolism
Zinc - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233
cites cdi_FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233
container_end_page 1141
container_issue 7
container_start_page 1137
container_title Biological & Pharmaceutical Bulletin
container_volume 28
creator Kubo, Satoshi
Fukuda, Hiroyuki
Ebara, Masaaki
Ikota, Nobuo
Saisho, Hiromitsu
Nakagawa, Hidehiko
Ozawa, Toshihiko
Yukawa, Masae
Kato, Kazuki
Satoh, Tsunenobu
Watayo, Takaho
Sakurai, Hiromu
description It has been reported that the copper (Cu) content of hepatocytes increases in chronic liver diseases and small hepatocellular carcinoma (HCC). In cells, Cu exists mainly as Cu-metallothionein (MT) or Cu, zinc (Zn)-superoxide dismutase (SOD). In this study, we investigated the biochemical state of Cu in the hepatocytes of patients with HCC using high-performance liquid chromatography (HPLC). The subjects of present study were 23 patients with HCC who underwent liver resection. The cancerous tissue and non-cancerous hepatic parenchyma with chronic disease were analyzed. In addition, as a normal control, hepatic tissue was collected at autopsy from 13 patients with no liver disease. Each sample was diluted with buffer, chilled, homogenized, and centrifuged. The supernatant was fractionated using HPLC. The metal contents of each fraction were measured using a desktop-type inductively coupled plasma (ICP) emission spectrochemical analyzer. HPLC analysis showed that MT existed mainly as Zn-MT in the normal hepatic tissue. The case of Cu,Zn-MT was significantly greater than Zn-MT in the non-cancerous, but diseased hepatic parenchyma than in the normal hepatic tissue (p
doi_str_mv 10.1248/bpb.28.1137
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68001944</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3121282171</sourcerecordid><originalsourceid>FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233</originalsourceid><addsrcrecordid>eNpdkV2L1DAUhoso7rp65b0EBFGkYz7aJr1TxnVHGHFA98abkKanMxnapJuki_sn_U2mM7MueJMQ3uc857Qny14SvCC0EB-asVlQsSCE8UfZOWEFz0tKysfZOa6JyCtSirPsWQh7jDHHlD3NzkhZ1xyL6jz7c3mr-klF4yxyHfpsQvSmmQ7vjYoRvA2ocx4t3TiCR8q26JexGhmLvkFUfe_iLsGQ3nP2Y0qU-21amF3DFFWAmV3uvLNGo7W5TZYUQQrCoWQFo4pOQ99PvUqNlNfGukGh62DsFq3MdpdvwKchBmU1JMXNZNqDcUiFW6_G3R16u9qsl--eZ0861Qd4cbovsusvlz-Xq3z9_err8tM615yxmGva0I6LpirqWlCtOAZRiRY6XDUlh5aTjrS0oqAa0F3JulYThUuSUFanX8gusjdH7-jdzQQhysGE-ROUBTcFWQmMSV0UCXz9H7h3k7dpNkmKomZFRQqcqPdHSnsXgodOjt4Myt9JguW8ZJmWLKmQ85IT_erknJoB2gf2tNUEXB2BlBqtemd7Y-Ghsw68Ma53kmJcSoypwDxd5KBPR0EI5qzms-nj0bQPUW3hXyvlo9E93I_Fj8eh_D7SO-UlWPYXrg7T4Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449346140</pqid></control><display><type>article</type><title>Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)</title><source>Full-Text Journals in Chemistry (Open access)</source><creator>Kubo, Satoshi ; Fukuda, Hiroyuki ; Ebara, Masaaki ; Ikota, Nobuo ; Saisho, Hiromitsu ; Nakagawa, Hidehiko ; Ozawa, Toshihiko ; Yukawa, Masae ; Kato, Kazuki ; Satoh, Tsunenobu ; Watayo, Takaho ; Sakurai, Hiromu</creator><creatorcontrib>Kubo, Satoshi ; Fukuda, Hiroyuki ; Ebara, Masaaki ; Ikota, Nobuo ; Saisho, Hiromitsu ; Nakagawa, Hidehiko ; Ozawa, Toshihiko ; Yukawa, Masae ; Kato, Kazuki ; Satoh, Tsunenobu ; Watayo, Takaho ; Sakurai, Hiromu ; School of Medicine ; dDepartment of Surgery ; cThe Second Department of Pathology ; Chiba University ; Kyoto Pharmaceutical University ; bNational Institute of Radiological Sciences ; eDepartment of Analytical and Bioinorganic Chemistry ; aFirst Department of Medicine ; Tokyo Metropolitan Ebara Hospital</creatorcontrib><description>It has been reported that the copper (Cu) content of hepatocytes increases in chronic liver diseases and small hepatocellular carcinoma (HCC). In cells, Cu exists mainly as Cu-metallothionein (MT) or Cu, zinc (Zn)-superoxide dismutase (SOD). In this study, we investigated the biochemical state of Cu in the hepatocytes of patients with HCC using high-performance liquid chromatography (HPLC). The subjects of present study were 23 patients with HCC who underwent liver resection. The cancerous tissue and non-cancerous hepatic parenchyma with chronic disease were analyzed. In addition, as a normal control, hepatic tissue was collected at autopsy from 13 patients with no liver disease. Each sample was diluted with buffer, chilled, homogenized, and centrifuged. The supernatant was fractionated using HPLC. The metal contents of each fraction were measured using a desktop-type inductively coupled plasma (ICP) emission spectrochemical analyzer. HPLC analysis showed that MT existed mainly as Zn-MT in the normal hepatic tissue. The case of Cu,Zn-MT was significantly greater than Zn-MT in the non-cancerous, but diseased hepatic parenchyma than in the normal hepatic tissue (p&lt;0.01). In comparison with non-cancerous hepatic parenchyma, the Cu-MT in the cancerous section was significantly greater than the Cu,Zn-MT (p&lt;0.01). The Cu content for MT was significantly higher in small HCC (&lt;40 mm) (p&lt;0.01), and the absence of Cu or Zn in the MT fraction was significantly more frequent in the large HCC (≥40 mm) (p&lt;0.01). The Cu and Zn content for SOD in the samples showed no significant difference. Increase in the Cu content in the cancerous hepatic tissue were, thought to be reflecting changes in the distribution of Cu in the MT fraction of hepatic tissues.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.28.1137</identifier><identifier>PMID: 15997086</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Aged ; Carcinoma, Hepatocellular - enzymology ; Carcinoma, Hepatocellular - metabolism ; Chromatography, High Pressure Liquid - methods ; Chronic Disease ; Copper - metabolism ; Female ; hepatocellular carcinoma ; high-performance liquid chromatography ; Humans ; Liver Neoplasms - enzymology ; Liver Neoplasms - metabolism ; Male ; metallothionein ; Metallothionein - chemistry ; Middle Aged ; particle-induced X-ray emission ; superoxide dismutase ; Superoxide Dismutase - metabolism ; Zinc - metabolism</subject><ispartof>Biological and Pharmaceutical Bulletin, 2005, Vol.28(7), pp.1137-1141</ispartof><rights>2005 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233</citedby><cites>FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15997086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kubo, Satoshi</creatorcontrib><creatorcontrib>Fukuda, Hiroyuki</creatorcontrib><creatorcontrib>Ebara, Masaaki</creatorcontrib><creatorcontrib>Ikota, Nobuo</creatorcontrib><creatorcontrib>Saisho, Hiromitsu</creatorcontrib><creatorcontrib>Nakagawa, Hidehiko</creatorcontrib><creatorcontrib>Ozawa, Toshihiko</creatorcontrib><creatorcontrib>Yukawa, Masae</creatorcontrib><creatorcontrib>Kato, Kazuki</creatorcontrib><creatorcontrib>Satoh, Tsunenobu</creatorcontrib><creatorcontrib>Watayo, Takaho</creatorcontrib><creatorcontrib>Sakurai, Hiromu</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>dDepartment of Surgery</creatorcontrib><creatorcontrib>cThe Second Department of Pathology</creatorcontrib><creatorcontrib>Chiba University</creatorcontrib><creatorcontrib>Kyoto Pharmaceutical University</creatorcontrib><creatorcontrib>bNational Institute of Radiological Sciences</creatorcontrib><creatorcontrib>eDepartment of Analytical and Bioinorganic Chemistry</creatorcontrib><creatorcontrib>aFirst Department of Medicine</creatorcontrib><creatorcontrib>Tokyo Metropolitan Ebara Hospital</creatorcontrib><title>Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)</title><title>Biological &amp; Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>It has been reported that the copper (Cu) content of hepatocytes increases in chronic liver diseases and small hepatocellular carcinoma (HCC). In cells, Cu exists mainly as Cu-metallothionein (MT) or Cu, zinc (Zn)-superoxide dismutase (SOD). In this study, we investigated the biochemical state of Cu in the hepatocytes of patients with HCC using high-performance liquid chromatography (HPLC). The subjects of present study were 23 patients with HCC who underwent liver resection. The cancerous tissue and non-cancerous hepatic parenchyma with chronic disease were analyzed. In addition, as a normal control, hepatic tissue was collected at autopsy from 13 patients with no liver disease. Each sample was diluted with buffer, chilled, homogenized, and centrifuged. The supernatant was fractionated using HPLC. The metal contents of each fraction were measured using a desktop-type inductively coupled plasma (ICP) emission spectrochemical analyzer. HPLC analysis showed that MT existed mainly as Zn-MT in the normal hepatic tissue. The case of Cu,Zn-MT was significantly greater than Zn-MT in the non-cancerous, but diseased hepatic parenchyma than in the normal hepatic tissue (p&lt;0.01). In comparison with non-cancerous hepatic parenchyma, the Cu-MT in the cancerous section was significantly greater than the Cu,Zn-MT (p&lt;0.01). The Cu content for MT was significantly higher in small HCC (&lt;40 mm) (p&lt;0.01), and the absence of Cu or Zn in the MT fraction was significantly more frequent in the large HCC (≥40 mm) (p&lt;0.01). The Cu and Zn content for SOD in the samples showed no significant difference. Increase in the Cu content in the cancerous hepatic tissue were, thought to be reflecting changes in the distribution of Cu in the MT fraction of hepatic tissues.</description><subject>Aged</subject><subject>Carcinoma, Hepatocellular - enzymology</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chronic Disease</subject><subject>Copper - metabolism</subject><subject>Female</subject><subject>hepatocellular carcinoma</subject><subject>high-performance liquid chromatography</subject><subject>Humans</subject><subject>Liver Neoplasms - enzymology</subject><subject>Liver Neoplasms - metabolism</subject><subject>Male</subject><subject>metallothionein</subject><subject>Metallothionein - chemistry</subject><subject>Middle Aged</subject><subject>particle-induced X-ray emission</subject><subject>superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Zinc - metabolism</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpdkV2L1DAUhoso7rp65b0EBFGkYz7aJr1TxnVHGHFA98abkKanMxnapJuki_sn_U2mM7MueJMQ3uc857Qny14SvCC0EB-asVlQsSCE8UfZOWEFz0tKysfZOa6JyCtSirPsWQh7jDHHlD3NzkhZ1xyL6jz7c3mr-klF4yxyHfpsQvSmmQ7vjYoRvA2ocx4t3TiCR8q26JexGhmLvkFUfe_iLsGQ3nP2Y0qU-21amF3DFFWAmV3uvLNGo7W5TZYUQQrCoWQFo4pOQ99PvUqNlNfGukGh62DsFq3MdpdvwKchBmU1JMXNZNqDcUiFW6_G3R16u9qsl--eZ0861Qd4cbovsusvlz-Xq3z9_err8tM615yxmGva0I6LpirqWlCtOAZRiRY6XDUlh5aTjrS0oqAa0F3JulYThUuSUFanX8gusjdH7-jdzQQhysGE-ROUBTcFWQmMSV0UCXz9H7h3k7dpNkmKomZFRQqcqPdHSnsXgodOjt4Myt9JguW8ZJmWLKmQ85IT_erknJoB2gf2tNUEXB2BlBqtemd7Y-Ghsw68Ma53kmJcSoypwDxd5KBPR0EI5qzms-nj0bQPUW3hXyvlo9E93I_Fj8eh_D7SO-UlWPYXrg7T4Q</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Kubo, Satoshi</creator><creator>Fukuda, Hiroyuki</creator><creator>Ebara, Masaaki</creator><creator>Ikota, Nobuo</creator><creator>Saisho, Hiromitsu</creator><creator>Nakagawa, Hidehiko</creator><creator>Ozawa, Toshihiko</creator><creator>Yukawa, Masae</creator><creator>Kato, Kazuki</creator><creator>Satoh, Tsunenobu</creator><creator>Watayo, Takaho</creator><creator>Sakurai, Hiromu</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050701</creationdate><title>Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)</title><author>Kubo, Satoshi ; Fukuda, Hiroyuki ; Ebara, Masaaki ; Ikota, Nobuo ; Saisho, Hiromitsu ; Nakagawa, Hidehiko ; Ozawa, Toshihiko ; Yukawa, Masae ; Kato, Kazuki ; Satoh, Tsunenobu ; Watayo, Takaho ; Sakurai, Hiromu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aged</topic><topic>Carcinoma, Hepatocellular - enzymology</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chronic Disease</topic><topic>Copper - metabolism</topic><topic>Female</topic><topic>hepatocellular carcinoma</topic><topic>high-performance liquid chromatography</topic><topic>Humans</topic><topic>Liver Neoplasms - enzymology</topic><topic>Liver Neoplasms - metabolism</topic><topic>Male</topic><topic>metallothionein</topic><topic>Metallothionein - chemistry</topic><topic>Middle Aged</topic><topic>particle-induced X-ray emission</topic><topic>superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kubo, Satoshi</creatorcontrib><creatorcontrib>Fukuda, Hiroyuki</creatorcontrib><creatorcontrib>Ebara, Masaaki</creatorcontrib><creatorcontrib>Ikota, Nobuo</creatorcontrib><creatorcontrib>Saisho, Hiromitsu</creatorcontrib><creatorcontrib>Nakagawa, Hidehiko</creatorcontrib><creatorcontrib>Ozawa, Toshihiko</creatorcontrib><creatorcontrib>Yukawa, Masae</creatorcontrib><creatorcontrib>Kato, Kazuki</creatorcontrib><creatorcontrib>Satoh, Tsunenobu</creatorcontrib><creatorcontrib>Watayo, Takaho</creatorcontrib><creatorcontrib>Sakurai, Hiromu</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>dDepartment of Surgery</creatorcontrib><creatorcontrib>cThe Second Department of Pathology</creatorcontrib><creatorcontrib>Chiba University</creatorcontrib><creatorcontrib>Kyoto Pharmaceutical University</creatorcontrib><creatorcontrib>bNational Institute of Radiological Sciences</creatorcontrib><creatorcontrib>eDepartment of Analytical and Bioinorganic Chemistry</creatorcontrib><creatorcontrib>aFirst Department of Medicine</creatorcontrib><creatorcontrib>Tokyo Metropolitan Ebara Hospital</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological &amp; Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kubo, Satoshi</au><au>Fukuda, Hiroyuki</au><au>Ebara, Masaaki</au><au>Ikota, Nobuo</au><au>Saisho, Hiromitsu</au><au>Nakagawa, Hidehiko</au><au>Ozawa, Toshihiko</au><au>Yukawa, Masae</au><au>Kato, Kazuki</au><au>Satoh, Tsunenobu</au><au>Watayo, Takaho</au><au>Sakurai, Hiromu</au><aucorp>School of Medicine</aucorp><aucorp>dDepartment of Surgery</aucorp><aucorp>cThe Second Department of Pathology</aucorp><aucorp>Chiba University</aucorp><aucorp>Kyoto Pharmaceutical University</aucorp><aucorp>bNational Institute of Radiological Sciences</aucorp><aucorp>eDepartment of Analytical and Bioinorganic Chemistry</aucorp><aucorp>aFirst Department of Medicine</aucorp><aucorp>Tokyo Metropolitan Ebara Hospital</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)</atitle><jtitle>Biological &amp; Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2005-07-01</date><risdate>2005</risdate><volume>28</volume><issue>7</issue><spage>1137</spage><epage>1141</epage><pages>1137-1141</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>It has been reported that the copper (Cu) content of hepatocytes increases in chronic liver diseases and small hepatocellular carcinoma (HCC). In cells, Cu exists mainly as Cu-metallothionein (MT) or Cu, zinc (Zn)-superoxide dismutase (SOD). In this study, we investigated the biochemical state of Cu in the hepatocytes of patients with HCC using high-performance liquid chromatography (HPLC). The subjects of present study were 23 patients with HCC who underwent liver resection. The cancerous tissue and non-cancerous hepatic parenchyma with chronic disease were analyzed. In addition, as a normal control, hepatic tissue was collected at autopsy from 13 patients with no liver disease. Each sample was diluted with buffer, chilled, homogenized, and centrifuged. The supernatant was fractionated using HPLC. The metal contents of each fraction were measured using a desktop-type inductively coupled plasma (ICP) emission spectrochemical analyzer. HPLC analysis showed that MT existed mainly as Zn-MT in the normal hepatic tissue. The case of Cu,Zn-MT was significantly greater than Zn-MT in the non-cancerous, but diseased hepatic parenchyma than in the normal hepatic tissue (p&lt;0.01). In comparison with non-cancerous hepatic parenchyma, the Cu-MT in the cancerous section was significantly greater than the Cu,Zn-MT (p&lt;0.01). The Cu content for MT was significantly higher in small HCC (&lt;40 mm) (p&lt;0.01), and the absence of Cu or Zn in the MT fraction was significantly more frequent in the large HCC (≥40 mm) (p&lt;0.01). The Cu and Zn content for SOD in the samples showed no significant difference. Increase in the Cu content in the cancerous hepatic tissue were, thought to be reflecting changes in the distribution of Cu in the MT fraction of hepatic tissues.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>15997086</pmid><doi>10.1248/bpb.28.1137</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0918-6158
ispartof Biological and Pharmaceutical Bulletin, 2005, Vol.28(7), pp.1137-1141
issn 0918-6158
1347-5215
language eng
recordid cdi_proquest_miscellaneous_68001944
source Full-Text Journals in Chemistry (Open access)
subjects Aged
Carcinoma, Hepatocellular - enzymology
Carcinoma, Hepatocellular - metabolism
Chromatography, High Pressure Liquid - methods
Chronic Disease
Copper - metabolism
Female
hepatocellular carcinoma
high-performance liquid chromatography
Humans
Liver Neoplasms - enzymology
Liver Neoplasms - metabolism
Male
metallothionein
Metallothionein - chemistry
Middle Aged
particle-induced X-ray emission
superoxide dismutase
Superoxide Dismutase - metabolism
Zinc - metabolism
title Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T14%3A50%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20Distribution%20Patterns%20for%20Copper%20and%20Zinc%20in%20Metallothionein%20and%20Superoxide%20Dismutase%20in%20Chronic%20Liver%20Diseases%20and%20Hepatocellular%20Carcinoma%20Using%20High-Performance%20Liquid%20Chromatography%20(HPLC)&rft.jtitle=Biological%20&%20Pharmaceutical%20Bulletin&rft.au=Kubo,%20Satoshi&rft.aucorp=School%20of%20Medicine&rft.date=2005-07-01&rft.volume=28&rft.issue=7&rft.spage=1137&rft.epage=1141&rft.pages=1137-1141&rft.issn=0918-6158&rft.eissn=1347-5215&rft_id=info:doi/10.1248/bpb.28.1137&rft_dat=%3Cproquest_cross%3E3121282171%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c733t-c2b2f78b649982ca70e868def06b57ed71f1d262eabecf53fdc1a0512ca390233%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1449346140&rft_id=info:pmid/15997086&rfr_iscdi=true