Protective Effects of Aucubin Isolated from Eucommia ulmoides against UVB-Induced Oxidative Stress in Human Skin Fibroblasts

Ultraviolet-B (UVB) irradiation has been demonstrated to produce reactive oxygen species (ROS) in the cells and skin, which induces the synthesis of matrix metalloproteinases (MMPs), causing skin photoaging. Using the human skin fibroblast HS68 cell line in the present study, we investigated the pho...

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Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2005, Vol.28(7), pp.1244-1248
Main Authors: Ho, Jin Nyoung, Lee, Yoo Hyun, Park, Jong Seok, Jun, Woo Jin, Kim, Hye Kyung, Hong, Bum Shik, Shin, Dong Hoon, Cho, Hong Yon
Format: Article
Language:English
Subjects:
aucubin
beta-Glucosidase - metabolism
Eucommia ulmoides
Eucommiaceae - chemistry
Fibroblasts - drug effects
Fibroblasts - enzymology
Fibroblasts - metabolism
Free Radicals
Glucosides - isolation & purification
Glucosides - pharmacology
Glutathione - metabolism
Humans
Hydrogen Peroxide - pharmacology
Iridoid Glucosides
Iridoids - isolation & purification
Iridoids - pharmacology
Matrix Metalloproteinase 1 - biosynthesis
Oxidative Stress
Penicillamine - analogs & derivatives
Penicillamine - pharmacology
photoaging
Skin - cytology
Skin - drug effects
Skin - enzymology
Skin - metabolism
ultraviolet B (UVB)
Ultraviolet Rays
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Summary:Ultraviolet-B (UVB) irradiation has been demonstrated to produce reactive oxygen species (ROS) in the cells and skin, which induces the synthesis of matrix metalloproteinases (MMPs), causing skin photoaging. Using the human skin fibroblast HS68 cell line in the present study, we investigated the photoprotective effects of aucubin from Eucommia ulmoides. Pretreatment with aucubin significantly inhibited the production of MMP-1 by 57% when compared to the UVB-irradiated cells. Additionally, the senescence-associated β-galactosidase (SA β-gal) activity was markedly decreased in the presence of aucubin, which indicates it as an antiphoto-induced aging compound. As the effect of aucubin was determined against ROS, the inhibited ROS formation and malondialdehyde (MDA) levels, and the increased cell viability and glutathione (GSH) level were observed with aucubin under UVB irradiation. Based upon these results, it was suggested that aucubin might play an important role in the cellular defense mechanism against UV radiation-induced photoaging. An understanding of the antioxidant properties of aucubin could, in part, act to elucidate its protective mechanism on the human skin photoaging.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.28.1244