Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20

Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentiall...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2006-05, Vol.6 (10), p.3154-3169
Main Authors: Boluyt, Marvin O., Brevick, Julie L., Rogers, David S., Randall, Michael J., Scalia, Antony F., Li, Zhao Bo
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Body Weight
Cell signaling
Differential proteomics
Electrophoresis, Gel, Two-Dimensional
Female
Fundamental and applied biological sciences. Psychology
Heart - anatomy & histology
HSP20 Heat-Shock Proteins - biosynthesis
Immunoblotting
Mass Spectrometry
Miscellaneous
Muscle Proteins - biosynthesis
Myocardium - metabolism
Organ Size
Phosphorylation
Physical Conditioning, Animal
Physiology
Proteins
Proteome - biosynthesis
Rats
Rats, Sprague-Dawley
Rats, Wistar
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Summary:Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Exercise‐trained rats underwent six weeks of progressive treadmill training five days/week, 0% grade, using an interval training protocol. Sedentary control rats were age‐ and weight‐matched to the exercise‐trained rats. Hearts were harvested at various times (0–72 h) after the last bout of exercise and were used to generate 2‐D electrophoretic proteome maps and immunoblots. Compared with hearts of sedentary rats, 26 protein spot intensities were significantly altered in hypertrophied hearts of exercise‐trained rats (p 
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200401356