Bone Marrow Stromal Cells Generate Muscle Cells and Repair Muscle Degeneration

• Published in: Science (American Association for the Advancement of Science) 2005-07, Vol.309 (5732), p.314-317
• Main Authors: Dezawa, Mari, Ishikawa, Hiroto, Itokazu, Yutaka, Yoshihara, Tomoyuki, Hoshino, Mikio, Takeda, Shin-ichi, Ide, Chizuka, Nabeshima, Yo-ichi
• Format: Article
• Language: English
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Applied cell therapy and gene therapy; Artificial satellites; Biological and medical sciences; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - physiology; Bone Marrow Transplantation; Cell Differentiation; Cell Fusion; Cell Lineage; Cell lines; Cell Proliferation; Cell Separation; Cell transplantation; Cells; Cells, Cultured; Colforsin - pharmacology; Dystonia; Ethics; Fibroblast Growth Factor 2 - pharmacology; Gene Expression Profiling; Hematopoietic stem cell transplantation; Hematopoietic stem cells; Homeodomain Proteins - analysis; Humans; Individualized Instruction; Logical Thinking; Medical disorders; Medical sciences; Mice; Mice, Inbred mdx; Mice, Nude; Muscle Cells - cytology; Muscle Development - genetics; Muscle diseases; Muscle Fibers, Skeletal - cytology; Muscle Proteins - analysis; Muscle, Skeletal - cytology; Muscles; Muscular diseases; Muscular Diseases - therapy; Muscular Dystrophy, Duchenne - therapy; Muscular system; Neuregulins - pharmacology; Neuroglia; Parkinson disease; Parkinson's disease; PAX7 Transcription Factor; Platelet-Derived Growth Factor - pharmacology; Rats; Regeneration; Satellite Cells, Skeletal Muscle - cytology; Satellite Cells, Skeletal Muscle - physiology; Skeletal muscle satellite cells; Stem cell transplantation; Stem cells; Stem Cells - cytology; Stem Cells - physiology; Stromal cells; Stromal Cells - cytology; Stromal Cells - physiology; Stromal Cells - transplantation; Transfection; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation; Transplants & implants
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1110364

Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage cells from human and rat general adherent MSCs with an efficiency of 89%. Induced cells differentiated into muscle fibers upon transplantation into degenerated muscles of rats and mdx-nude mice. The induced population contained Pax7-positive cells that contributed to subsequent regeneration of muscle upon repetitive damage without additional transplantation of cells. These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow.