Anti-Transglutaminase Antibody Assay of the Culture Medium of Intestinal Biopsy Specimens Can Improve the Accuracy of Celiac Disease Diagnosis

We measured anti-transglutaminase (anti-tTG) antibody in the culture medium of intestinal biopsy specimens from patients with suspected celiac disease (CD) and evaluated the relationship between antibody production and severity of intestinal mucosal damage. We performed diagnostic testing for CD on...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2006-06, Vol.52 (6), p.1175-1180
Main Authors: Carroccio, Antonio, Di Prima, Lidia, Pirrone, Giuseppe, Scalici, Calogero, Florena, Ada M, Gasparin, Maurizio, Tolazzi, Giuseppe, Gucciardi, Antonino, Sciume, Carmelo, Iacono, Giuseppe
Format: Article
Language:English
Subjects:
Accuracy
Adolescent
Adult
Aged
Aged, 80 and over
Analytical, structural and metabolic biochemistry
Antibodies
Antibody Formation
Atrophy
Autoantibodies - analysis
Autoantibodies - blood
Autoimmune diseases
Biological and medical sciences
Biopsy
Celiac disease
Celiac Disease - diagnosis
Child
Child, Preschool
Culture Media
Duodenum - immunology
Duodenum - pathology
Female
Fundamental and applied biological sciences. Psychology
Gastroenterology
Gluten
Humans
Immunoglobulin G - analysis
Immunoglobulin G - blood
Infant
Intestinal Mucosa - immunology
Intestinal Mucosa - pathology
Investigative techniques, diagnostic techniques (general aspects)
Lymphocytes
Male
Medical sciences
Middle Aged
Muscle Fibers, Skeletal - immunology
Nutrition research
Patients
Pediatrics
Predictive Value of Tests
Prospective Studies
Serology
Transglutaminases - immunology
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Summary:We measured anti-transglutaminase (anti-tTG) antibody in the culture medium of intestinal biopsy specimens from patients with suspected celiac disease (CD) and evaluated the relationship between antibody production and severity of intestinal mucosal damage. We performed diagnostic testing for CD on 273 consecutive patients. In addition to routine histologic evaluation of duodenal biopsy specimens, we assayed anti-tTG antibodies in serum and in the culture medium of duodenal biopsy specimens. CD was diagnosed in 191 of the 273 patients. Sensitivity and specificity of the serum anti-endomysium (EmA) and anti-tTG assays were 83% and 85% and 99% and 95%, respectively, and both had 88% diagnostic accuracy. EmA and anti-tTG assayed in the culture medium had 98% sensitivity, 100% specificity, and 98% diagnostic accuracy (vs serum assays; P
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2005.061366