Kinase-regulated quantal assemblies and kiss-and-run recycling of caveolae

A functional genomics approach has revealed that caveolae/raft-mediated endocytosis is subject to regulation by a large number of kinases. Here we explore the role of some of these kinases in caveolae dynamics. We discover that caveolae operate using principles different from classical membrane traf...

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Bibliographic Details
Published in:Nature 2005-07, Vol.436 (7047), p.128-133
Main Authors: Zerial, Marino, Pelkmans, Lucas
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Transport
Caveolae - chemistry
Caveolae - enzymology
Caveolae - metabolism
Caveolin 1
Caveolins - deficiency
Caveolins - genetics
Caveolins - metabolism
Cell Line
Cell physiology
Endocytosis
Enzymes
Fibroblasts
Fundamental and applied biological sciences. Psychology
Genomics
HeLa Cells
Humanities and Social Sciences
Humans
letter
Membrane Fusion
Mice
Molecular and cellular biology
Molecular structure
multidisciplinary
Protein Kinases - metabolism
Science
Science (multidisciplinary)
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Summary:A functional genomics approach has revealed that caveolae/raft-mediated endocytosis is subject to regulation by a large number of kinases. Here we explore the role of some of these kinases in caveolae dynamics. We discover that caveolae operate using principles different from classical membrane trafficking. First, each caveolar coat contains a set number (one 'quantum') of caveolin-1 molecules. Second, caveolae are either stored as in stationary multi-caveolar structures at the plasma membrane, or undergo continuous cycles of fission and fusion with the plasma membrane in a small volume beneath the surface, without disassembling the caveolar coat. Third, a switch mechanism shifts caveolae from this localized cycle to long-range cytoplasmic transport. We have identified six kinases that regulate different steps of the caveolar cycle. Our observations reveal new principles in caveolae trafficking and suggest that the dynamic properties of caveolae and their transport competence are regulated by different kinases operating at several levels.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature03866