Differential gene expression in Perillus bioculatus nymphs fed a suboptimal artificial diet

• Published in: Journal of insect physiology 2006-06, Vol.52 (6), p.586-592
• Main Authors: Yocum, G.D., Coudron, T.A., Brandt, S.L.
• Format: Article
• Language: English
• Subjects: Animal Nutritional Physiological Phenomena; Animals; Artificial diet; artificial diets; Biomarkers; chitin-binding protein gene; Developmental; developmental gene expression; developmental stages; Gene Expression; gene expression regulation; Gene Expression Regulation, Developmental; Gene Library; gene up-regulation; Generational; generational gene expression; Genes, Insect; Heteroptera - genetics; Heteroptera - growth & development; Heteroptera - metabolism; insect development; insect generations; insect nutrition; insect rearing; Nutrigenomics; nutritional status; nutritive value; Nymph - genetics; Nymph - growth & development; Nymph - metabolism; Pentatomidae; Perillus bioculatus; predatory insects; tyrosine-3-monooxygenase gene
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2006.02.006

Fragments of two artificial diet up-regulated and two prey up-regulated transcripts were isolated from the predatory Pentatomid Perillus bioculatus using suppression subtractive hybridization. A BlastX search found similarities for two diet-upregulated clones, i.e., the tyrosine-3-monooxygenase gene and the gene for the chitin binding protein, Gasp. The probe generated from the tyrosine-3-monooxygenase clone hybridized to two transcripts 2.3 and 1.2 kb in size. The two transcripts were differentially regulated: the 2.3 kb transcript was upregulated in the first and late third instar diet-fed nymphs, whereas the 1.2 kb transcript was upregulated in the second and early third instar diet-fed nymphs. The Gasp gene was upregulated in late third instar nymphs. A positive correlation was found between levels of expression of the isolated genes and the number of generations the insects had been reared on the artificial diet.