Single-Molecule Fluorescence Spectroscopy of Protein Folding

Single‐molecule spectroscopy is an important new approach for studying the intrinsically heterogeneous process of protein folding. This Review illustrates how different single‐molecule fluorescence techniques have improved our understanding of mechanistic aspects in protein folding, exemplified by a...

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Bibliographic Details
Published in:Chemphyschem 2005-07, Vol.6 (7), p.1206-1220
Main Author: Schuler, Benjamin
Format: Article
Language:English
Subjects:
Biological and medical sciences
Conformational dynamics in molecular biology
emission spectroscopy
FRET (fluorescence resonance energy transfer)
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Protein Folding
proteins
Proteins - chemistry
Sensitivity and Specificity
single-molecule studies
Spectrometry, Fluorescence - methods
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Summary:Single‐molecule spectroscopy is an important new approach for studying the intrinsically heterogeneous process of protein folding. This Review illustrates how different single‐molecule fluorescence techniques have improved our understanding of mechanistic aspects in protein folding, exemplified by a series of recent experiments on a small protein. Unraveling nature's secrets: Protein folding is a process fundamental to all forms of life we know. Single‐molecule fluorescence spectroscopy is a promising approach for studying the mechanisms involved, and the first steps of such studies are described in this Review. The figure shows a small protein, labeled with chromophores suitable for Förster resonance energy transfer measurements.
ISSN:1439-4235
1439-7641
DOI:10.1002/cphc.200400609