Novel Agmatine-Containing Poly(amidoamine) Hydrogels as Scaffolds for Tissue Engineering

Novel biocompatible and biodegradable amphoteric poly(amidoamine) (PAA) hydrogels were designed for applications as scaffolds for tissue engineering. These hydrogels (PAA-AG1 and PAA-AG2) were obtained by polyaddition of 2,2-bisacrylamidoacetic acid with 2-methylpiperazine and 4-aminobutyl guanidine...

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Bibliographic Details
Published in:Biomacromolecules 2005-07, Vol.6 (4), p.2229-2235
Main Authors: Ferruti, Paolo, Bianchi, Sabrina, Ranucci, Elisabetta, Chiellini, Federica, Piras, Anna Maria
Format: Article
Language:English
Subjects:
3T3 Cells
Agmatine - chemistry
Animals
Applied sciences
Biological and medical sciences
Cell Adhesion
Cell Proliferation
Exact sciences and technology
Hydrogels - chemistry
Medical sciences
Mice
Mice, Inbred BALB C
Nylons - chemistry
Organic polymers
Physicochemistry of polymers
Polyamines - chemistry
Polycondensation
Preparation, kinetics, thermodynamics, mechanism and catalysts
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Tissue Engineering
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Summary:Novel biocompatible and biodegradable amphoteric poly(amidoamine) (PAA) hydrogels were designed for applications as scaffolds for tissue engineering. These hydrogels (PAA-AG1 and PAA-AG2) were obtained by polyaddition of 2,2-bisacrylamidoacetic acid with 2-methylpiperazine and 4-aminobutyl guanidine, a bioactive molecule with a known ability to induce adhesion to cell membranes. They contain carboxylic functions in their main chain and interchain connections deriving from two different cross-linking agents:  for PAA-AG1, a multifunctional primary amine, that is, 1,10-decanediamine; for PAA-AG2, a purposely synthesized PAA (PAA-NH2) containing pendant NH2. Both PAA-AG1 and PAA-AG2 proved noncytotoxic and adhesive to cell membranes, as ascertained by means of cytotoxicity and proliferation tests carried out on fibroblast cell lines. Good apparent mechanical strength was also observed in the case of PAA-AG2, cross-linked with the PAA-NH2. Both PAA-AG1 and PAA-AG2 underwent degradation tests under controlled conditions simulating the biological environments, that is, Dulbecco medium at pH 7.4 and 37 °C. They completely dissolved within 10 and about 40 days, respectively. In both cases, the degradation products were completely noncytotoxic. All the results of this paper point to the conclusion that agmatine-based PAA hydrogels are excellent substrates for cell proliferation.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm050210+