Bacterial cell shape-dependent inflammatory response in mammary epithelial cells

An in vitro co-culture model of SCp2 mammary epithelial cells and Escherichia coli strains was established in bacterial non-CO(2) incubators. Co-culturing SCp2 cells with either the rod-shaped W3110 or spherical-shaped GC7378Tn10 strains of Escherichia coli led to an increase in interleukin-6 (IL-6)...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2006-06, Vol.52 (6), p.424-429
Main Authors: Khattar, Medhat M, Bazzi, Samer, Kogan, Joanna, Talhouk, Rabih S
Format: Article
Language:English
Subjects:
Animals
Bacteria
Cell Line
Coculture Techniques
E coli
Epithelial Cells - immunology
Epithelial Cells - microbiology
Escherichia coli
Escherichia coli - physiology
Female
Incubators
Interleukin-6 - metabolism
Mammary Glands, Animal - cytology
Mammary Glands, Animal - immunology
Mammary Glands, Animal - microbiology
Mice
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An in vitro co-culture model of SCp2 mammary epithelial cells and Escherichia coli strains was established in bacterial non-CO(2) incubators. Co-culturing SCp2 cells with either the rod-shaped W3110 or spherical-shaped GC7378Tn10 strains of Escherichia coli led to an increase in interleukin-6 (IL-6) levels by SCp2 cells after 9 h. At a ratio of 1:100 (epithelial:bacterial), the rod-shaped W3110 strain induced almost double the amount of IL-6 induced by the spherical-shaped GC7378Tn10 strain. The effect of Escherichia coli morphology (rod versus spherical) on IL-6 production by SCp2 cells was further investigated by shifting GC7378Tn10 morphology to rod through introducing the pbpA gene by transduction and transformation. In both approaches, the generated rod strains elicited higher IL-6 levels in SCp2 cells compared to the spherical ones at 1:50 and 1:100 ratios (epithelial:bacterial). Our findings demonstrate the significance of cell shape in bacterial-host interactions with potential implications in bacterial pathogenesis in general.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-005-0100-4