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Genetic profiling of aortic allografts: prothymosin alpha as potential target?
Summary Transplant arteriosclerosis is the result of intima proliferation in large vessels upon organ transplantation. Obliteration of the vascular lumen will ultimately lead to ischemia and late graft failure. Gene array analysis was performed to identify factors involved in the pathogenesis of tra...
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| Published in: | Transplant international 2005-08, Vol.18 (8), p.1010-1015 |
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| Main Authors: | , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c4237-c5552ae378a5ccbb61a4547bfb08cb77ea92cd03d7ce2ca9261b7f1374a5ff773 |
| container_end_page | 1015 |
| container_issue | 8 |
| container_start_page | 1010 |
| container_title | Transplant international |
| container_volume | 18 |
| creator | Joosten, Simone A. Smit van Dixhoorn, Mieneke G. A. Borrias, Maria C. Ham, Vanessa Groot Koerkamp, Marian J. A. Savolainen‐Peltonen, Hanna M. Häyry, Pekka Daha, Mohamed R. Kooten, Cees Paul, Leendert C. |
| description | Summary
Transplant arteriosclerosis is the result of intima proliferation in large vessels upon organ transplantation. Obliteration of the vascular lumen will ultimately lead to ischemia and late graft failure. Gene array analysis was performed to identify factors involved in the pathogenesis of transplant arteriosclerosis. Aortic transplants from Dark Agouti to Wistar Furth rats were performed to identify potential target genes. Hierarchical clustering of genes specifically upregulated in allogeneic but not in syngeneic aortas revealed 19 genes. A gene that fulfilled these criteria is prothymosin alpha (PTMA), a regulator of estrogen receptor transcriptional activity. PTMA gene and protein expression levels were confirmed by PCR and immunohistochemistry. Estrogen receptor staining was increased in allogeneic aortas. Furthermore, cyclin D1 a downstream target of PTMA, was also up regulated in allogeneic aortas. In conclusion, PTMA was identified as potential candidate gene involved in transplant arteriosclerosis. |
| doi_str_mv | 10.1111/j.1432-2277.2005.00157.x |
| format | article |
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Transplant arteriosclerosis is the result of intima proliferation in large vessels upon organ transplantation. Obliteration of the vascular lumen will ultimately lead to ischemia and late graft failure. Gene array analysis was performed to identify factors involved in the pathogenesis of transplant arteriosclerosis. Aortic transplants from Dark Agouti to Wistar Furth rats were performed to identify potential target genes. Hierarchical clustering of genes specifically upregulated in allogeneic but not in syngeneic aortas revealed 19 genes. A gene that fulfilled these criteria is prothymosin alpha (PTMA), a regulator of estrogen receptor transcriptional activity. PTMA gene and protein expression levels were confirmed by PCR and immunohistochemistry. Estrogen receptor staining was increased in allogeneic aortas. Furthermore, cyclin D1 a downstream target of PTMA, was also up regulated in allogeneic aortas. In conclusion, PTMA was identified as potential candidate gene involved in transplant arteriosclerosis.</description><identifier>ISSN: 0934-0874</identifier><identifier>EISSN: 1432-2277</identifier><identifier>DOI: 10.1111/j.1432-2277.2005.00157.x</identifier><identifier>PMID: 16008753</identifier><language>eng</language><publisher>Oxford, UK: Munksgaard International Publishers</publisher><subject>Animals ; Aorta - metabolism ; Aorta - transplantation ; Arteriosclerosis - etiology ; Biological and medical sciences ; Cyclin D1 - analysis ; estrogen receptor ; gene array ; Gene Expression Profiling ; General aspects ; Immunohistochemistry ; Male ; Medical sciences ; Nephrology. Urinary tract diseases ; Pharmacology. Drug treatments ; Polymerase Chain Reaction ; Protein Precursors - analysis ; Protein Precursors - genetics ; prothymosin alpha ; rat aorta transplantation ; Rats ; Rats, Inbred WF ; Thymosin - analogs & derivatives ; Thymosin - analysis ; Thymosin - genetics ; transplant arteriosclerosis ; Transplantation, Homologous</subject><ispartof>Transplant international, 2005-08, Vol.18 (8), p.1010-1015</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Ltd. Aug 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4237-c5552ae378a5ccbb61a4547bfb08cb77ea92cd03d7ce2ca9261b7f1374a5ff773</citedby><cites>FETCH-LOGICAL-c4237-c5552ae378a5ccbb61a4547bfb08cb77ea92cd03d7ce2ca9261b7f1374a5ff773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17355010$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16008753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joosten, Simone A.</creatorcontrib><creatorcontrib>Smit van Dixhoorn, Mieneke G. A.</creatorcontrib><creatorcontrib>Borrias, Maria C.</creatorcontrib><creatorcontrib>Ham, Vanessa</creatorcontrib><creatorcontrib>Groot Koerkamp, Marian J. A.</creatorcontrib><creatorcontrib>Savolainen‐Peltonen, Hanna M.</creatorcontrib><creatorcontrib>Häyry, Pekka</creatorcontrib><creatorcontrib>Daha, Mohamed R.</creatorcontrib><creatorcontrib>Kooten, Cees</creatorcontrib><creatorcontrib>Paul, Leendert C.</creatorcontrib><title>Genetic profiling of aortic allografts: prothymosin alpha as potential target?</title><title>Transplant international</title><addtitle>Transpl Int</addtitle><description>Summary
Transplant arteriosclerosis is the result of intima proliferation in large vessels upon organ transplantation. Obliteration of the vascular lumen will ultimately lead to ischemia and late graft failure. Gene array analysis was performed to identify factors involved in the pathogenesis of transplant arteriosclerosis. Aortic transplants from Dark Agouti to Wistar Furth rats were performed to identify potential target genes. Hierarchical clustering of genes specifically upregulated in allogeneic but not in syngeneic aortas revealed 19 genes. A gene that fulfilled these criteria is prothymosin alpha (PTMA), a regulator of estrogen receptor transcriptional activity. PTMA gene and protein expression levels were confirmed by PCR and immunohistochemistry. Estrogen receptor staining was increased in allogeneic aortas. Furthermore, cyclin D1 a downstream target of PTMA, was also up regulated in allogeneic aortas. In conclusion, PTMA was identified as potential candidate gene involved in transplant arteriosclerosis.</description><subject>Animals</subject><subject>Aorta - metabolism</subject><subject>Aorta - transplantation</subject><subject>Arteriosclerosis - etiology</subject><subject>Biological and medical sciences</subject><subject>Cyclin D1 - analysis</subject><subject>estrogen receptor</subject><subject>gene array</subject><subject>Gene Expression Profiling</subject><subject>General aspects</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Precursors - analysis</subject><subject>Protein Precursors - genetics</subject><subject>prothymosin alpha</subject><subject>rat aorta transplantation</subject><subject>Rats</subject><subject>Rats, Inbred WF</subject><subject>Thymosin - analogs & derivatives</subject><subject>Thymosin - analysis</subject><subject>Thymosin - genetics</subject><subject>transplant arteriosclerosis</subject><subject>Transplantation, Homologous</subject><issn>0934-0874</issn><issn>1432-2277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkN1qGzEQRkVpaNy0r1CWQnO3m5G0snZLIITQ_EBIoKTXYlaWbBl55UhrEr99tbWpIVfRjaSZM8PHIaSgUNF8zpYVrTkrGZOyYgCiAqBCVq8fyOR_4yOZQMvrEhpZH5PPKS0BgDUCPpFjOoVcFnxCHm5Mbwani3UM1nnXz4tgCwxxrKH3YR7RDunn2B8W21VIrs_19QILTMU6DKYfHPpiwDg3w8UXcmTRJ_N1f5-QP9e_nq5uy_vHm7ury_tS14zLUgshGBouGxRad92UYi1q2dkOGt1JabBlegZ8JrVhOn-mtJOWclmjsFZKfkJOd3tzrOeNSYNauaSN99ibsElq2gAD3rYZ_P4GXIZN7HM2xWgrGuAAGWp2kI4hpWisWke3wrhVFNQoXC3V6FWNXtUoXP0Trl7z6Lf9_k23MrPD4N5wBn7sAUwavY3Ya5cOnORCAB0znO-4F-fN9t0B1NPvu_zgfwGo45ty</recordid><startdate>200508</startdate><enddate>200508</enddate><creator>Joosten, Simone A.</creator><creator>Smit van Dixhoorn, Mieneke G. A.</creator><creator>Borrias, Maria C.</creator><creator>Ham, Vanessa</creator><creator>Groot Koerkamp, Marian J. A.</creator><creator>Savolainen‐Peltonen, Hanna M.</creator><creator>Häyry, Pekka</creator><creator>Daha, Mohamed R.</creator><creator>Kooten, Cees</creator><creator>Paul, Leendert C.</creator><general>Munksgaard International Publishers</general><general>Blackwell Publishing</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200508</creationdate><title>Genetic profiling of aortic allografts: prothymosin alpha as potential target?</title><author>Joosten, Simone A. ; Smit van Dixhoorn, Mieneke G. A. ; Borrias, Maria C. ; Ham, Vanessa ; Groot Koerkamp, Marian J. A. ; Savolainen‐Peltonen, Hanna M. ; Häyry, Pekka ; Daha, Mohamed R. ; Kooten, Cees ; Paul, Leendert C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4237-c5552ae378a5ccbb61a4547bfb08cb77ea92cd03d7ce2ca9261b7f1374a5ff773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Aorta - metabolism</topic><topic>Aorta - transplantation</topic><topic>Arteriosclerosis - etiology</topic><topic>Biological and medical sciences</topic><topic>Cyclin D1 - analysis</topic><topic>estrogen receptor</topic><topic>gene array</topic><topic>Gene Expression Profiling</topic><topic>General aspects</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Pharmacology. Drug treatments</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Precursors - analysis</topic><topic>Protein Precursors - genetics</topic><topic>prothymosin alpha</topic><topic>rat aorta transplantation</topic><topic>Rats</topic><topic>Rats, Inbred WF</topic><topic>Thymosin - analogs & derivatives</topic><topic>Thymosin - analysis</topic><topic>Thymosin - genetics</topic><topic>transplant arteriosclerosis</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joosten, Simone A.</creatorcontrib><creatorcontrib>Smit van Dixhoorn, Mieneke G. A.</creatorcontrib><creatorcontrib>Borrias, Maria C.</creatorcontrib><creatorcontrib>Ham, Vanessa</creatorcontrib><creatorcontrib>Groot Koerkamp, Marian J. A.</creatorcontrib><creatorcontrib>Savolainen‐Peltonen, Hanna M.</creatorcontrib><creatorcontrib>Häyry, Pekka</creatorcontrib><creatorcontrib>Daha, Mohamed R.</creatorcontrib><creatorcontrib>Kooten, Cees</creatorcontrib><creatorcontrib>Paul, Leendert C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplant international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joosten, Simone A.</au><au>Smit van Dixhoorn, Mieneke G. A.</au><au>Borrias, Maria C.</au><au>Ham, Vanessa</au><au>Groot Koerkamp, Marian J. A.</au><au>Savolainen‐Peltonen, Hanna M.</au><au>Häyry, Pekka</au><au>Daha, Mohamed R.</au><au>Kooten, Cees</au><au>Paul, Leendert C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic profiling of aortic allografts: prothymosin alpha as potential target?</atitle><jtitle>Transplant international</jtitle><addtitle>Transpl Int</addtitle><date>2005-08</date><risdate>2005</risdate><volume>18</volume><issue>8</issue><spage>1010</spage><epage>1015</epage><pages>1010-1015</pages><issn>0934-0874</issn><eissn>1432-2277</eissn><abstract>Summary
Transplant arteriosclerosis is the result of intima proliferation in large vessels upon organ transplantation. Obliteration of the vascular lumen will ultimately lead to ischemia and late graft failure. Gene array analysis was performed to identify factors involved in the pathogenesis of transplant arteriosclerosis. Aortic transplants from Dark Agouti to Wistar Furth rats were performed to identify potential target genes. Hierarchical clustering of genes specifically upregulated in allogeneic but not in syngeneic aortas revealed 19 genes. A gene that fulfilled these criteria is prothymosin alpha (PTMA), a regulator of estrogen receptor transcriptional activity. PTMA gene and protein expression levels were confirmed by PCR and immunohistochemistry. Estrogen receptor staining was increased in allogeneic aortas. Furthermore, cyclin D1 a downstream target of PTMA, was also up regulated in allogeneic aortas. In conclusion, PTMA was identified as potential candidate gene involved in transplant arteriosclerosis.</abstract><cop>Oxford, UK</cop><pub>Munksgaard International Publishers</pub><pmid>16008753</pmid><doi>10.1111/j.1432-2277.2005.00157.x</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0934-0874 |
| ispartof | Transplant international, 2005-08, Vol.18 (8), p.1010-1015 |
| issn | 0934-0874 1432-2277 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | Animals Aorta - metabolism Aorta - transplantation Arteriosclerosis - etiology Biological and medical sciences Cyclin D1 - analysis estrogen receptor gene array Gene Expression Profiling General aspects Immunohistochemistry Male Medical sciences Nephrology. Urinary tract diseases Pharmacology. Drug treatments Polymerase Chain Reaction Protein Precursors - analysis Protein Precursors - genetics prothymosin alpha rat aorta transplantation Rats Rats, Inbred WF Thymosin - analogs & derivatives Thymosin - analysis Thymosin - genetics transplant arteriosclerosis Transplantation, Homologous |
| title | Genetic profiling of aortic allografts: prothymosin alpha as potential target? |
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