Proteomic analysis of liver tissues subjected to early ischemia/reperfusion injury during human orthotopic liver transplantation

Knowledge of early molecular events occurring upon ischemia/reperfusion (I/R) during liver transplantation (LT) is of great importance to improve the therapeutic intervention of surgical treatment. However, nowadays, few data are available on early protein targets of I/R injury. To identify these pr...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2006-06, Vol.6 (11), p.3455-3465
Main Authors: Vascotto, Carlo, Cesaratto, Laura, D'Ambrosio, Chiara, Scaloni, Andrea, Avellini, Claudio, Paron, Igor, Baccarani, Umberto, Adani, Gian Luigi, Tiribelli, Claudio, Quadrifoglio, Franco, Tell, Gianluca
Format: Article
Language:English
Subjects:
Adult
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Electrophoresis, Gel, Two-Dimensional
Female
Fundamental and applied biological sciences. Psychology
Hepatocytes
Humans
Liver - blood supply
Liver - chemistry
Liver - pathology
Liver Transplantation
Male
Middle Aged
Miscellaneous
Oxidative Stress
Proteins
Proteome - analysis
Reperfusion Injury - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Knowledge of early molecular events occurring upon ischemia/reperfusion (I/R) during liver transplantation (LT) is of great importance to improve the therapeutic intervention of surgical treatment. However, nowadays, few data are available on early protein targets of I/R injury. To identify these proteins, we used a differential proteomics approach in the characterization human liver biopsies during I/R upon LT. Analyses were performed on nine donor livers during LT. By using 2‐DE and MALDI‐TOF MS, we identified 36 proteins which resulted significantly altered upon I/R injury. The majority of these proteins are functionally involved in lipid and energy metabolism, in different metabolic pathways, in redox signalling and in oxidative‐stress response. Our data represent the first global approach in the study of I/R injury in liver.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200500770