Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets

Mesenchymal cells in the developing pancreas express the neural stem cell marker nestin and the transcription factor islet-1 (Isl-1). Using defined culture conditions we isolated on a single cell basis nestin producing cells from human pancreatic islets. These cells were immortalized with lentiviral...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-07, Vol.345 (3), p.1167-1176
Main Authors: Eberhardt, Michael, Salmon, Patrick, von Mach, Marc-Alexander, Hengstler, Jan Georg, Brulport, Marc, Linscheid, Philippe, Seboek, Dalma, Oberholzer, José, Barbero, Andrea, Martin, Ivan, Müller, Beat, Trono, Didier, Zulewski, Henryk
Format: Article
Language:English
Subjects:
ABCG2
Adipocytes - metabolism
Albumin
Albumins - metabolism
ATP Binding Cassette Transporter, Sub-Family G, Member 2
ATP-Binding Cassette Transporters - metabolism
Cell Culture Techniques - methods
Cell Differentiation
Diabetes
Differentiation
Homeodomain Proteins - physiology
Human
Humans
Insulin
Intermediate Filament Proteins - physiology
Isl-1
Islets of Langerhans - cytology
Islets of Langerhans - metabolism
Lentivirus - metabolism
LIM-Homeodomain Proteins
Mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Neoplasm Proteins - metabolism
Nerve Tissue Proteins - physiology
Nestin
Neurons - metabolism
Osteoblasts - metabolism
Pancreatic islet
Transcription Factors
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Summary:Mesenchymal cells in the developing pancreas express the neural stem cell marker nestin and the transcription factor islet-1 (Isl-1). Using defined culture conditions we isolated on a single cell basis nestin producing cells from human pancreatic islets. These cells were immortalized with lentiviral vectors coding for telomerase and mBmi. They are positive for Isl-1 and nestin and have the potential to adopt a pancreatic endocrine phenotype with expression of critical transcription factors including Ipf-1, Isl-1, Ngn-3, Pax4, Pax6, Nkx2.2, and Nkx6.1 as well as the islet hormones insulin, glucagon, and somatostatin. In addition, they can be differentiated into human albumin producing cells in vivo when grafted into a SCID mouse liver. In accordance with a mesenchymal phenotype, the cells were also able to adopt adipocytic or osteocytic phenotypes in vitro. In conclusion, cultured pancreatic islets contain nestin and Isl-1 positive mesenchymal stem cells with multipotential developmental capacity.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.05.016