General Contribution of Nonspecific Interactions to Fluorescence Intensity

Many chemical compounds, including nonfluorescent ones, induce changes in the fluorescence spectra of certain probes, such as berberine cation and Reichardt's betaine, both in the absence and the presence of solvent, that affect almost exclusively emission intensity. In this work, the applicati...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2006-06, Vol.78 (11), p.3699-3705
Main Authors: Gálvez, Eva M, Matt, Muriel, Cebolla, Vicente L, Fernandes, Francisco, Membrado, Luis, Cossío, Fernando P, Garriga, Rosa, Vela, Jesús, Guermouche, M. Hassan
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemical compounds
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Exact sciences and technology
Fluorescence
Other chromatographic methods
Solvents
Spectrometric and optical methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Many chemical compounds, including nonfluorescent ones, induce changes in the fluorescence spectra of certain probes, such as berberine cation and Reichardt's betaine, both in the absence and the presence of solvent, that affect almost exclusively emission intensity. In this work, the application of fluorescence detection by intensity changes (FDIC) to HPLC and TLC chromatographic systems with fluorescence detectors has been studied. FDIC detection is of special interest in detecting nonfluorescent analytes, either in HPLC or in TLC mode. It does not involve covalent interactions, and the dielectric permittivity (ε) of the medium plays an important role. The balance between nonspecific and specific interactions produces either an increase or a decrease in fluorescence intensity. Therefore, the influence of chromatographic conditions and chemical structure of analytes on the sign and magnitude of fluorescence peaks for sample detection in HPLC and TLC systems has been discussed. In general, probe nature and concentration determine response and detection sensitivity for a given sample in TLC and HPLC. As solubility and fluorescence properties in solvents determine the operating conditions for a FDIC probe in HPLC mode, nature and flows of mobile phase and solvent are important for chromatographic response and detection sensitivity.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac058045b