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The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity
Primate lentiviruses such as human immunodeficiency type 1 (HIV-1) have the capacity to infect non-dividing cells such as tissue macrophages. In the process, viral complementary DNA traverses the nuclear envelope to integrate within chromatin. Given the intimate association between chromatin and the...
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| Published in: | Nature 2006-06, Vol.441 (7093), p.641-645 |
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| cites | cdi_FETCH-LOGICAL-c697t-fbcf24d8cb700130d4cfd14f2ded9c0cb0dd843532697c290973c57e347f39a83 |
| container_end_page | 645 |
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| container_title | Nature |
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| creator | Stevenson, Mario Jacque, Jean-Marc |
| description | Primate lentiviruses such as human immunodeficiency type 1 (HIV-1) have the capacity to infect non-dividing cells such as tissue macrophages. In the process, viral complementary DNA traverses the nuclear envelope to integrate within chromatin. Given the intimate association between chromatin and the nuclear envelope, we examined whether HIV-1 appropriates nuclear envelope components during infection. Here we show that emerin, an integral inner-nuclear-envelope protein, is necessary for HIV-1 infection. Infection of primary macrophages lacking emerin was abortive in that viral cDNA localized to the nucleus but integration into chromatin was inefficient, and conversion of viral cDNA to non-functional episomal cDNA increased. HIV-1 cDNA associated with emerin in vivo, and the interaction of viral cDNA with chromatin was dependent on emerin. Barrier-to-autointegration factor (BAF), the LEM (LAP, emerin, MAN) binding partner of emerin, was required for the association of viral cDNA with emerin and for the ability of emerin to support virus infection. Therefore emerin, which bridges the interface between the inner nuclear envelope and chromatin, may be necessary for chromatin engagement by viral cDNA before integration. |
| doi_str_mv | 10.1038/nature04682 |
| format | article |
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Therefore emerin, which bridges the interface between the inner nuclear envelope and chromatin, may be necessary for chromatin engagement by viral cDNA before integration.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/nature04682</identifier><identifier>PMID: 16680152</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Biological and medical sciences ; Cells, Cultured ; Chromatin - genetics ; Chromatin - metabolism ; Deoxyribonucleic acid ; Development and progression ; DNA ; DNA, Complementary - genetics ; DNA, Complementary - metabolism ; DNA, Viral - genetics ; DNA, Viral - metabolism ; DNA-Binding Proteins - deficiency ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Fundamental and applied biological sciences. 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In the process, viral complementary DNA traverses the nuclear envelope to integrate within chromatin. Given the intimate association between chromatin and the nuclear envelope, we examined whether HIV-1 appropriates nuclear envelope components during infection. Here we show that emerin, an integral inner-nuclear-envelope protein, is necessary for HIV-1 infection. Infection of primary macrophages lacking emerin was abortive in that viral cDNA localized to the nucleus but integration into chromatin was inefficient, and conversion of viral cDNA to non-functional episomal cDNA increased. HIV-1 cDNA associated with emerin in vivo, and the interaction of viral cDNA with chromatin was dependent on emerin. Barrier-to-autointegration factor (BAF), the LEM (LAP, emerin, MAN) binding partner of emerin, was required for the association of viral cDNA with emerin and for the ability of emerin to support virus infection. Therefore emerin, which bridges the interface between the inner nuclear envelope and chromatin, may be necessary for chromatin engagement by viral cDNA before integration.</description><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chromatin - genetics</subject><subject>Chromatin - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>Development and progression</subject><subject>DNA</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Complementary - metabolism</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - metabolism</subject><subject>DNA-Binding Proteins - deficiency</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells</subject><subject>HIV</subject><subject>HIV infection</subject><subject>HIV Integrase - metabolism</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - physiology</subject><subject>Human immunodeficiency virus</subject><subject>Human immunodeficiency virus 1</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>letter</subject><subject>Macrophages - cytology</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - virology</subject><subject>Membrane Proteins - deficiency</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Microbiology</subject><subject>multidisciplinary</subject><subject>Nuclear Envelope - metabolism</subject><subject>Nuclear Proteins - deficiency</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Thymopoietins - deficiency</subject><subject>Thymopoietins - genetics</subject><subject>Thymopoietins - metabolism</subject><subject>Viral envelopes</subject><subject>Viral proteins</subject><subject>Virology</subject><subject>Virus Integration - physiology</subject><issn>0028-0836</issn><issn>1476-4687</issn><issn>1476-4679</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqF0s9v0zAUB3ALgVgpnLijsgkkBBnPP2I7x2karNIkJBhcLdd5KZlSp7OTif33uEpFWxSpysGR8_Gz8_Uj5DWFcwpcf_a26wOCkJo9IRMqlMzSu3pKJgBMZ6C5PCEvYrwDgJwq8ZycUCk10JxNyNXtb5zV3mPIfO8atCFD_4BNu8bZOrQd1n6GKwxpCLjsG9thnF3Pf2U0rarQdfVD3T2-JM8q20R8tR2n5OeXq9vL6-zm29f55cVN5mShuqxauIqJUruFAqAcSuGqkoqKlVgWDtwCylILnnOWuGMFFIq7XCEXquKF1XxK3g9109Hue4ydWdXRYdNYj20fTfornhbLo5BLpkRe0KOQaQo508VxSClXuji-NVVUiwIgwdP_4F3bB5_yMwxELqhOSUzJ2YCWtkGTIm-7YN2mormQnKWEJOdJZSNqielabdN6rOo0feBPR7xb1_dmH52PoPSUuKrdaNUPBwuS6fBPt7R9jGb-4_uh_ThYF9oYA1ZmHeqVDY-Ggtm0tdlr66TfbKPqFyssd3bbxwm82wIbnW2qYL2r484pTXPJN9f9aXAxffJLDLvMx_d9O_Bh8l-9ffMXzNsVUQ</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Stevenson, Mario</creator><creator>Jacque, Jean-Marc</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7TG</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>S0X</scope><scope>SOI</scope><scope>7U5</scope><scope>L7M</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope></search><sort><creationdate>20060601</creationdate><title>The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity</title><author>Stevenson, Mario ; 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Academic</collection><jtitle>Nature</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stevenson, Mario</au><au>Jacque, Jean-Marc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity</atitle><jtitle>Nature</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>441</volume><issue>7093</issue><spage>641</spage><epage>645</epage><pages>641-645</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><eissn>1476-4679</eissn><coden>NATUAS</coden><abstract>Primate lentiviruses such as human immunodeficiency type 1 (HIV-1) have the capacity to infect non-dividing cells such as tissue macrophages. In the process, viral complementary DNA traverses the nuclear envelope to integrate within chromatin. Given the intimate association between chromatin and the nuclear envelope, we examined whether HIV-1 appropriates nuclear envelope components during infection. Here we show that emerin, an integral inner-nuclear-envelope protein, is necessary for HIV-1 infection. Infection of primary macrophages lacking emerin was abortive in that viral cDNA localized to the nucleus but integration into chromatin was inefficient, and conversion of viral cDNA to non-functional episomal cDNA increased. HIV-1 cDNA associated with emerin in vivo, and the interaction of viral cDNA with chromatin was dependent on emerin. Barrier-to-autointegration factor (BAF), the LEM (LAP, emerin, MAN) binding partner of emerin, was required for the association of viral cDNA with emerin and for the ability of emerin to support virus infection. Therefore emerin, which bridges the interface between the inner nuclear envelope and chromatin, may be necessary for chromatin engagement by viral cDNA before integration.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>16680152</pmid><doi>10.1038/nature04682</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature, 2006-06, Vol.441 (7093), p.641-645 |
| issn | 0028-0836 1476-4687 1476-4679 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68033266 |
| source | North East Research Libraries Nature Academic Titles |
| subjects | Biological and medical sciences Cells, Cultured Chromatin - genetics Chromatin - metabolism Deoxyribonucleic acid Development and progression DNA DNA, Complementary - genetics DNA, Complementary - metabolism DNA, Viral - genetics DNA, Viral - metabolism DNA-Binding Proteins - deficiency DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology HeLa Cells HIV HIV infection HIV Integrase - metabolism HIV-1 - genetics HIV-1 - physiology Human immunodeficiency virus Human immunodeficiency virus 1 Humanities and Social Sciences Humans letter Macrophages - cytology Macrophages - metabolism Macrophages - virology Membrane Proteins - deficiency Membrane Proteins - genetics Membrane Proteins - metabolism Microbiology multidisciplinary Nuclear Envelope - metabolism Nuclear Proteins - deficiency Nuclear Proteins - genetics Nuclear Proteins - metabolism Physiological aspects Proteins Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Science Science (multidisciplinary) Thymopoietins - deficiency Thymopoietins - genetics Thymopoietins - metabolism Viral envelopes Viral proteins Virology Virus Integration - physiology |
| title | The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T03%3A09%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20inner-nuclear-envelope%20protein%20emerin%20regulates%20HIV-1%20infectivity&rft.jtitle=Nature&rft.au=Stevenson,%20Mario&rft.date=2006-06-01&rft.volume=441&rft.issue=7093&rft.spage=641&rft.epage=645&rft.pages=641-645&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/nature04682&rft_dat=%3Cgale_proqu%3EA632097633%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c697t-fbcf24d8cb700130d4cfd14f2ded9c0cb0dd843532697c290973c57e347f39a83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=204541835&rft_id=info:pmid/16680152&rft_galeid=A632097633&rfr_iscdi=true |