Improving Access to the Control Region and tRNA Gene Clusters of Dipteran Mitochondrial DNA

Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gen...

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Bibliographic Details
Published in:Journal of medical entomology 2006-05, Vol.43 (3), p.636-639
Main Authors: OLIVEIRA, M. T., DA ROSA, A. C., AZEREDO-ESPIN, A.M.L., LESSINGER, A. C.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
conserved primers
control region
Diptera
Diptera - genetics
DNA Primers
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
Fundamental and applied biological sciences. Psychology
genes
genetic techniques and protocols
insect pests
Insecta
Invertebrates
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
mitochondrial DNA
nucleotide sequences
Polymerase Chain Reaction
RNA, Transfer - genetics
Sequence Alignment
Sequence Analysis, DNA
SHORT COMMUNICATIONS
transfer RNA
tRNAs
Vectors. Intermediate hosts
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Summary:Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gene clusters of dipterans of medical and veterinary importance, including Muscidae, Calliphoridae, and Oestridae species. These new primers were useful for investigating the nucleotide information and the structural organization of dipteran mtDNA.
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585%282006%2943%5B636%3AIATTCR%5D2.0.CO%3B2