The usefulness of CD71 expression by flow cytometry for differentiating indolent from aggressive CD10+ B-cell lymphomas

We studied 34 low- and 30 high-grade CD10+ B-cell lymphomas. Forward light scatter (FSC) and CD71 fluorescence intensity (CD71i) of tumor cells were measured and normalized by corresponding values for resting T cells. Significant differences in CD71i values between low- and high-grade lymphomas were...

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Bibliographic Details
Published in:American journal of clinical pathology 2006-07, Vol.126 (1), p.39-46
Main Authors: WU, Julie M, BOROWITZ, Michael J, WEIR, Edward G
Format: Article
Language:English
Subjects:
Antigens, CD - metabolism
Biological and medical sciences
Biomarkers, Tumor - metabolism
Diagnosis, Differential
Flow Cytometry - instrumentation
Flow Cytometry - methods
Hematologic and hematopoietic diseases
Humans
Immunophenotyping - methods
Investigative techniques, diagnostic techniques (general aspects)
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Light
Lymphoma, B-Cell - classification
Lymphoma, B-Cell - metabolism
Lymphoma, B-Cell - pathology
Medical sciences
Neprilysin - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Receptors, Transferrin - metabolism
Retrospective Studies
ROC Curve
Scattering, Radiation
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Summary:We studied 34 low- and 30 high-grade CD10+ B-cell lymphomas. Forward light scatter (FSC) and CD71 fluorescence intensity (CD71i) of tumor cells were measured and normalized by corresponding values for resting T cells. Significant differences in CD71i values between low- and high-grade lymphomas were observed by the Mann-Whitney U test (P < .001) and receiver operating characteristic (ROC) curve analysis (P < .001). FSC was not significantly different between low- and high-grade lymphomas; the area under the ROC curve was less than that for CD71i. Neither FSC nor CD71i significantly differentiated follicular lymphoma (FL) grades. A comparison of all FLs (grades 1-3) and non-FL high-grade lymphomas (Burkitt lymphoma [BL] and large B-cell lymphoma [LBCL]) showed significant differences in CD71i (P < .001) and FSC (P = .021). Differences were significant in CD71i and FSC between FL and LBCL (P < .001) but not between FL and BL. CD71i is more potent than FSC for distinguishing CD10+ low- from high-grade lymphomas and FL from non-FL high-grade lymphomas. Sensitivity and specificity are limited owing to inability to identify FL3. In ROC analysis, a high value for CD71i can identify BL and LBCL with a high degree of certainty.
ISSN:0002-9173
1943-7722
DOI:10.1309/x8lbrrbbc43pl9hc