The effects of estradiol on estrogen receptor and glutamate transporter expression in organotypic hippocampal cultures exposed to oxygen--glucose deprivation

The molecular basis of estrogen-mediated neuroprotection against brain ischemia remains unclear. In the present study, we investigated changes in expression of estrogen receptors (ERs) alpha and beta and excitatory amino acid transporters (EAAT) 1 and 2 in rat organotypic hippocampal slice cultures...

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Published in:Neurochemical research 2006-04, Vol.31 (4), p.483-490
Main Authors: Cimarosti, Helena, O'Shea, Ross D, Jones, Nicole M, Horn, Ana Paula, Simão, Fabrício, Zamin, Lauren L, Nassif, Melissa, Frozza, Rudimar, Netto, Carlos Alexandre, Beart, Philip M, Salbego, Christianne
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Estradiol - metabolism
Estrogen Receptor alpha - metabolism
Estrogen Receptor beta - metabolism
Excitatory Amino Acid Transporter 1 - metabolism
Excitatory Amino Acid Transporter 2 - metabolism
Glucose - metabolism
Hippocampus - cytology
Hippocampus - metabolism
Hypoxia
Male
Rats
Rats, Wistar
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Summary:The molecular basis of estrogen-mediated neuroprotection against brain ischemia remains unclear. In the present study, we investigated changes in expression of estrogen receptors (ERs) alpha and beta and excitatory amino acid transporters (EAAT) 1 and 2 in rat organotypic hippocampal slice cultures treated with estradiol and subsequently exposed to oxygen--glucose deprivation (OGD). Pretreatment with 17beta-estradiol (10 nM) for 7 days protected the CA1 area of hippocampus against OGD (60 min), reducing cellular injury by 46% compared to the vehicle control group. Levels of ERalpha protein were significantly reduced by 20% after OGD in both vehicle- and estradiol-treated cultures, whereas ERbeta was significantly up-regulated by 25% in the estradiol-treated cultures. In contrast, EAAT1 and EAAT2 levels were unchanged in response to estradiol treatment in this model of OGD. These findings suggest that estrogen-induced neuroprotection against ischemia might involve regulation of ERbeta and, consequently, of the genes influenced by this receptor.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-006-9043-9