CHLORORESPIRATORY REDUCTION 6 Is a Novel Factor Required for Accumulation of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis

The chloroplast NAD(P)H dehydrogenase (NDH) complex is involved in photosystem I cyclic electron transport and chlororespiration in higher plants. An Arabidopsis (Arabidopsis thaliana) chlororespiratory reduction 6 (crr6) mutant lacking NDH activity was identified by means of chlorophyll fluorescenc...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2006-06, Vol.141 (2), p.737-744
Main Authors: Munshi, M. Kamruzzaman, Kobayashi, Yoshichika, Shikanai, Toshiharu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - enzymology
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Base Sequence
Bioenergetics and Photosynthesis
Biological and medical sciences
Chlamydomonas reinhardtii
Chlorophylls
Chloroplasts
Chloroplasts - enzymology
Cloning, Molecular
Cyanobacteria
Dehydrogenases
DNA Primers
Enzymes
Fluorescence
Fundamental and applied biological sciences. Psychology
Genomics
Metabolism
Molecular Sequence Data
NADPH Dehydrogenase - metabolism
Photons
Plant physiology and development
Plants
Proteins
Sequence Homology, Amino Acid
Thylakoids
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Summary:The chloroplast NAD(P)H dehydrogenase (NDH) complex is involved in photosystem I cyclic electron transport and chlororespiration in higher plants. An Arabidopsis (Arabidopsis thaliana) chlororespiratory reduction 6 (crr6) mutant lacking NDH activity was identified by means of chlorophyll fluorescence imaging. Accumulation of the NDH complex was impaired in crr6. Physiological characterization of photosynthetic electron transport indicated the specific defect of the NDH complex in crr6. In contrast to the CRR7 protein that was recently identified as a potential novel subunit of the NDH complex by means of the same screening, the CRR6 protein was stable under the crr2 mutant background in which the NDH complex does not accumulate. The CRR6 gene (At2g47910) encodes a novel protein without any known motif. Although CRR6 does not have any transmembrane domains, it is localized in the thylakoid membrane fraction of the chloroplast. CRR6 is conserved in phototrophs, including cyanobacteria, from which the chloroplast NDH complex has evolutionally originated, but not in Chlamydomonas reinhardtii, in which the NDH complex is absent. We believe that CRR6 is a novel specific factor for the assembly or stabilization of the NDH complex.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.106.080267