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Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people

Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigat...

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Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2006-06, Vol.74 (6), p.347-356
Main Authors: Alnajjar, A., Chabane Sari, D., Abuharfeil, N., Hudaib, M., Aburjai, T.
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container_title Prostaglandins, leukotrienes and essential fatty acids
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description Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigated their effect on lymphoproliferation response, the concentration of interleukin 2 produced in each essay and phospholipid fatty acid composition of lymphocyte membrane. Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics. On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions. Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic.
doi_str_mv 10.1016/j.plefa.2006.03.001
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subjects Arachidonic Acid - pharmacology
Biological and medical sciences
Cell Proliferation - drug effects
Diabetes Mellitus, Type 2 - blood
Diabetes. Impaired glucose tolerance
Docosahexaenoic Acids - pharmacology
Eicosapentaenoic Acid - pharmacology
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Fatty Acids, Omega-3 - pharmacology
Fatty Acids, Omega-6 - pharmacology
Female
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Insulin - pharmacology
Interleukins - biosynthesis
Interleukins - metabolism
Jordan
Male
Medical sciences
Membrane Lipids - chemistry
Phospholipids - chemistry
Subcellular Fractions
T-Lymphocytes - drug effects
Vertebrates: endocrinology
title Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people
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