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Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people
Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigat...
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Published in: | Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2006-06, Vol.74 (6), p.347-356 |
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description | Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigated their effect on lymphoproliferation response, the concentration of interleukin 2 produced in each essay and phospholipid fatty acid composition of lymphocyte membrane.
Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics.
On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions.
Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic. |
doi_str_mv | 10.1016/j.plefa.2006.03.001 |
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Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics.
On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions.
Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic.</description><identifier>ISSN: 0952-3278</identifier><identifier>EISSN: 1532-2823</identifier><identifier>DOI: 10.1016/j.plefa.2006.03.001</identifier><identifier>PMID: 16650969</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Arachidonic Acid - pharmacology ; Biological and medical sciences ; Cell Proliferation - drug effects ; Diabetes Mellitus, Type 2 - blood ; Diabetes. Impaired glucose tolerance ; Docosahexaenoic Acids - pharmacology ; Eicosapentaenoic Acid - pharmacology ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Fatty Acids, Omega-3 - pharmacology ; Fatty Acids, Omega-6 - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Insulin - pharmacology ; Interleukins - biosynthesis ; Interleukins - metabolism ; Jordan ; Male ; Medical sciences ; Membrane Lipids - chemistry ; Phospholipids - chemistry ; Subcellular Fractions ; T-Lymphocytes - drug effects ; Vertebrates: endocrinology</subject><ispartof>Prostaglandins, leukotrienes and essential fatty acids, 2006-06, Vol.74 (6), p.347-356</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-b01ef9e5bb3c95723c453348d9bc3c1a6ac40420cb66985bf0a024f61bdf78bc3</citedby><cites>FETCH-LOGICAL-c302t-b01ef9e5bb3c95723c453348d9bc3c1a6ac40420cb66985bf0a024f61bdf78bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17878906$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16650969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alnajjar, A.</creatorcontrib><creatorcontrib>Chabane Sari, D.</creatorcontrib><creatorcontrib>Abuharfeil, N.</creatorcontrib><creatorcontrib>Hudaib, M.</creatorcontrib><creatorcontrib>Aburjai, T.</creatorcontrib><title>Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people</title><title>Prostaglandins, leukotrienes and essential fatty acids</title><addtitle>Prostaglandins Leukot Essent Fatty Acids</addtitle><description>Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigated their effect on lymphoproliferation response, the concentration of interleukin 2 produced in each essay and phospholipid fatty acid composition of lymphocyte membrane.
Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics.
On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions.
Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic.</description><subject>Arachidonic Acid - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation - drug effects</subject><subject>Diabetes Mellitus, Type 2 - blood</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Docosahexaenoic Acids - pharmacology</subject><subject>Eicosapentaenoic Acid - pharmacology</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>Fatty Acids, Omega-6 - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Insulin - pharmacology</subject><subject>Interleukins - biosynthesis</subject><subject>Interleukins - metabolism</subject><subject>Jordan</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Lipids - chemistry</subject><subject>Phospholipids - chemistry</subject><subject>Subcellular Fractions</subject><subject>T-Lymphocytes - drug effects</subject><subject>Vertebrates: endocrinology</subject><issn>0952-3278</issn><issn>1532-2823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kc-O0zAQxiMEYrsLT4CEfIETKWM7cZMDB7Ra_mklLnC2HHusurhxsJ2V8oS8Fk5bacWFgzWS_fu-Gc9XVa8obClQ8f6wnTxatWUAYgt8C0CfVBvaclazjvGn1Qb6ltWc7bqr6jqlAwAwSpvn1RUVooVe9Jvqz521qDMJlow1J2o0pQoyBb_MY1J5jiqjIVblvBClnUkkjMQvx2kf9JKRTDF4Z7FgLozviBszRo_zLzeuT2bW6_3JtyhSOd5N7l9DHY5TSO4EFlleJiSMGKcGzE6ftHtUPu8XkubhUMZNK_ctRKNKFwxlDy-qZ1b5hC8v9ab6-enux-2X-v7756-3H-9rzYHlegCKtsd2GLju2x3jumk5bzrTD5prqoTSDTQM9CBE37WDBQWssYIOxu66wtxUb8--5XO_Z0xZHl3S6L0aMcxJig4a3lNRQH4GdQwpRbRyiu6o4iIpyDU_eZCn_OSanwQuS35F9fpiPw9HNI-aS2AFeHMBVNLK26hG7dIjt-t2XQ9r-w9nDssyHhxGmbTDUaNxsSxQmuD-O8hfRXO--Q</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Alnajjar, A.</creator><creator>Chabane Sari, D.</creator><creator>Abuharfeil, N.</creator><creator>Hudaib, M.</creator><creator>Aburjai, T.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200606</creationdate><title>Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people</title><author>Alnajjar, A. ; Chabane Sari, D. ; Abuharfeil, N. ; Hudaib, M. ; Aburjai, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-b01ef9e5bb3c95723c453348d9bc3c1a6ac40420cb66985bf0a024f61bdf78bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Arachidonic Acid - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation - drug effects</topic><topic>Diabetes Mellitus, Type 2 - blood</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Docosahexaenoic Acids - pharmacology</topic><topic>Eicosapentaenoic Acid - pharmacology</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>Fatty Acids, Omega-6 - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Insulin - pharmacology</topic><topic>Interleukins - biosynthesis</topic><topic>Interleukins - metabolism</topic><topic>Jordan</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Lipids - chemistry</topic><topic>Phospholipids - chemistry</topic><topic>Subcellular Fractions</topic><topic>T-Lymphocytes - drug effects</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alnajjar, A.</creatorcontrib><creatorcontrib>Chabane Sari, D.</creatorcontrib><creatorcontrib>Abuharfeil, N.</creatorcontrib><creatorcontrib>Hudaib, M.</creatorcontrib><creatorcontrib>Aburjai, T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prostaglandins, leukotrienes and essential fatty acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alnajjar, A.</au><au>Chabane Sari, D.</au><au>Abuharfeil, N.</au><au>Hudaib, M.</au><au>Aburjai, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people</atitle><jtitle>Prostaglandins, leukotrienes and essential fatty acids</jtitle><addtitle>Prostaglandins Leukot Essent Fatty Acids</addtitle><date>2006-06</date><risdate>2006</risdate><volume>74</volume><issue>6</issue><spage>347</spage><epage>356</epage><pages>347-356</pages><issn>0952-3278</issn><eissn>1532-2823</eissn><abstract>Dietary lipid manipulation may affect a great number of immune parameters, such as lymphocyte proliferation, cytokine synthesis. In this study, lymphocytes of diabetic type 2 were incubated with different polyunsaturated fatty acid (docosahexaenoic, eicosapentaenoic, arachidonic acid) for investigated their effect on lymphoproliferation response, the concentration of interleukin 2 produced in each essay and phospholipid fatty acid composition of lymphocyte membrane.
Our results found that the concanavalin A and insulin increase significantly the proliferative response while eicosapentaenoic, arachidonic and docosahexaenoic acid inhibited that by different degrees: 47%, 37% and 19%, respectively, for healthy subjects and 39%, 29% and 13% for diabetes. However, the concentration of IL-2 produced in presence of either docosahexaenoic, eicosapentaenoic or arachidonic acid was significantly reduced by 36%, 32% and 39%, respectively, in controls while 16%, 15% and 23%, respectively, in diabetics.
On the other hand, the tested fatty acids demonstrated a major impact on the fatty acid composition of different phospholipid fractions of lymphocyte membrane but these fractions were different in their response to each fatty acid examined. For instance, the addition of docosahexaenoic acid to culture media was accompanied with a predominant composition of docosahexaenoic acid in phospholipid fractions.
Also, our results showed a notable increased proportion of arachidonic, eicosapentaenoic and docosahexaenoic acids in control phospholipid fractions than those of diabetic.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>16650969</pmid><doi>10.1016/j.plefa.2006.03.001</doi><tpages>10</tpages></addata></record> |
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subjects | Arachidonic Acid - pharmacology Biological and medical sciences Cell Proliferation - drug effects Diabetes Mellitus, Type 2 - blood Diabetes. Impaired glucose tolerance Docosahexaenoic Acids - pharmacology Eicosapentaenoic Acid - pharmacology Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Fatty Acids, Omega-3 - pharmacology Fatty Acids, Omega-6 - pharmacology Female Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Insulin - pharmacology Interleukins - biosynthesis Interleukins - metabolism Jordan Male Medical sciences Membrane Lipids - chemistry Phospholipids - chemistry Subcellular Fractions T-Lymphocytes - drug effects Vertebrates: endocrinology |
title | Effect of n-3 and n-6 polyunsaturated fatty acids on lymphocyte proliferation, interleukin production and phospholipid fatty acids composition in type 2 diabetic and healthy subjects in Jordan people |
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