Patterning the heart, a template for human cardiomyocyte development

Although in mice, the dynamics of gene expression during heart development is well characterized, information on humans is scarce due to the limited availability of material. Here, we analyzed the transcriptional distribution of Mlc‐2a, Mlc‐1v, Mlc‐2v, and atrial natriuretic factor (ANF) in human em...

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Bibliographic Details
Published in:Developmental dynamics 2006-07, Vol.235 (7), p.1994-2002
Main Authors: Chuva de Sousa Lopes, Susana M., Hassink, Rutger J., Feijen, Alie, van Rooijen, Marga A., Doevendans, Pieter A., Tertoolen, Leon, Brutel de la Rivière, Aart, Mummery, Christine L.
Format: Article
Language:English
Subjects:
Animals
Atrial Natriuretic Factor - metabolism
Cardiomegaly - metabolism
Cardiomegaly - pathology
cardiomyocyte
Cells, Cultured
Electrophysiology
embryo
Gene Expression Regulation, Developmental
heart
Heart - anatomy & histology
Heart - embryology
Heart - growth & development
human
Humans
Mice
Myocardial Infarction - metabolism
Myocardial Infarction - pathology
Myocardium - cytology
Myocardium - metabolism
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
Myocytes, Cardiac - physiology
myosin light chain
Myosin Light Chains - metabolism
Protein Isoforms - metabolism
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Summary:Although in mice, the dynamics of gene expression during heart development is well characterized, information on humans is scarce due to the limited availability of material. Here, we analyzed the transcriptional distribution of Mlc‐2a, Mlc‐1v, Mlc‐2v, and atrial natriuretic factor (ANF) in human embryonic hearts between 7 and 18 weeks of gestation and in healthy and hypertrophic adult hearts by in situ hybridization and compared expression with that in mice. Strikingly, Mlc‐2a, Mlc‐1v, and ANF, which are essentially chamber‐restricted in mice by mid‐gestation, showed a broader distribution in humans. On the other hand, Mlc‐2v may prove to be an adequate ventricular marker in humans in contrast to mouse. This study emphasizes the importance of careful comparative human–animal analyses during embryonic development and adulthood, as avoiding erroneous extrapolations may be critical to develop new and successful myocardial replacement therapies. Development Dynamics 235:1994–2002, 2006. © 2006 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.20830