In vivo optical imaging of human adenoid cystic carcinoma cell metastasis

A noninvasive, whole-body, real-time fluorescence optical imaging of stable high-level green fluorescent protein (GFP)—expressing human adenoid cystic carcinoma (ACC-M-GFP) was demonstrated for in vivo visualization of metastatic behavior in nude mice. Five-week-old female nude mice were injected wi...

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Bibliographic Details
Published in:Oral oncology 2005-08, Vol.41 (7), p.709-715
Main Authors: Xiong, Tao, Zhang, Zhihong, Liu, Bi-Feng, Zeng, Shaoqun, Chen, Yanping, Chu, Jun, Luo, Qingming
Format: Article
Language:English
Subjects:
Adenoid cystic carcinoma (ACC-M)
Animals
Biological and medical sciences
Carcinoma, Adenoid Cystic - secondary
Carcinoma, Squamous Cell - secondary
Cell Proliferation
Female
Green fluorescent protein (GFP)
Green Fluorescent Proteins
In vivo
Luminescent Agents
Lung Neoplasms - secondary
Medical sciences
Metastasis
Mice
Mice, Inbred BALB C
Mice, Nude
Microscopy, Fluorescence
Neoplasm Transplantation
Optical imaging
Otorhinolaryngology. Stomatology
Tumors
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Summary:A noninvasive, whole-body, real-time fluorescence optical imaging of stable high-level green fluorescent protein (GFP)—expressing human adenoid cystic carcinoma (ACC-M-GFP) was demonstrated for in vivo visualization of metastatic behavior in nude mice. Five-week-old female nude mice were injected with ACC-M-GFP in the primary organ: submandibular gland. Metastases were only visualized by GFP expression in the lung. However, metastatic lesions of ACC-M-GFP in the lung, muscle, bladder and bony were found by imaging of GFP expression in intact mice through tail vein injection of ACC-M-GFP cells. The construction of highly fluorescent and stable GFP transfectants of ACC-M has revealed the multi-organ metastatic capability of ACC-M cells through this optical imaging.
ISSN:1368-8375
1879-0593
DOI:10.1016/j.oraloncology.2005.03.012