Nongastric Helicobacter Species Detected in the Intestinal Tract of Children

The presence of members of the family Helicobacteraceae in the colon of children was investigated using PCR, denaturing gradient gel electrophoresis, and fluorescent in situ hybridization. The rate of detection of species belonging to the Helicobacteraceae family in children with inflammatory bowel...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-06, Vol.44 (6), p.2276-2279
Main Authors: Zhang, Li, Day, Andrew, McKenzie, Gavin, Mitchell, Hazel
Format: Article
Language:English
Subjects:
Adolescent
Bacteriology
Child
Child, Preschool
Colon - microbiology
Electrophoresis - methods
Female
Gram-Negative Bacterial Infections - microbiology
Helicobacter
Helicobacter - genetics
Helicobacter - isolation & purification
Helicobacter Infections - microbiology
Humans
In Situ Hybridization, Fluorescence
Inflammatory Bowel Diseases - microbiology
Male
Polymerase Chain Reaction
Wolinella - genetics
Wolinella - isolation & purification
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Summary:The presence of members of the family Helicobacteraceae in the colon of children was investigated using PCR, denaturing gradient gel electrophoresis, and fluorescent in situ hybridization. The rate of detection of species belonging to the Helicobacteraceae family in children with inflammatory bowel disease (92%) and irritable bowel syndrome (100%) was found to be significantly higher than that in healthy children (25%) (P < 0.05).
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.02017-05