Total Oxidant Scavenging Capacity of Euterpe oleracea Mart. (Açaí) Seeds and Identification of Their Polyphenolic Compounds

The antioxidant capacity of methanol and ethanol seed extracts from Euterpe oleracea Mart. (açaí) against the reactive oxygen species (ROS) peroxyl radicals, peroxynitrite, and hydroxyl radicals was studied with the total oxidant scavenging capacity (TOSC) assay in a modified and automated version....

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-06, Vol.54 (12), p.4162-4167
Main Authors: Rodrigues, Roberta B, Lichtenthäler, Ramona, Zimmermann, Benno F, Papagiannopoulos, Menelaos, Fabricius, Heinz, Marx, Friedhelm, Maia, José G. S, Almeida, Ossalin
Format: Article
Language:English
Subjects:
antioxidant activity
antioxidants
Antioxidants - analysis
Antioxidants - pharmacology
Arecaceae - chemistry
Biological and medical sciences
Chromatography, High Pressure Liquid
epicatechin
Euterpe oleracea
Flavonoids - analysis
Food industries
Free Radical Scavengers
free radicals
Fundamental and applied biological sciences. Psychology
Hydroxyl Radical
hydroxyl radicals
peroxyl radicals
peroxynitrite
Peroxynitrous Acid
Phenols - analysis
plant extracts
Polyphenols
procyanidins
protocatechuic acid
reactive oxygen species
seeds
Seeds - chemistry
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Summary:The antioxidant capacity of methanol and ethanol seed extracts from Euterpe oleracea Mart. (açaí) against the reactive oxygen species (ROS) peroxyl radicals, peroxynitrite, and hydroxyl radicals was studied with the total oxidant scavenging capacity (TOSC) assay in a modified and automated version. Cold methanol digestion was the most efficient extraction method with respect to the antioxidant capacity. The extracts exhibit good antioxidant capacity against peroxyl radicals, similar to the capacity of the pulp. The antioxidant capacity against peroxynitrite and hydroxyl radicals is even higher. The main antioxidants identified by HPLC-MS and HPLC-CEAD are five different procyanidins (di- through pentamers); furthermore, protocatechuic acid and epicatechin were identified as minor compounds. Determination of TOSC values of HPLC seed extract fractions indicates that the procyanidins contribute substantially to the overall antioxidant capacity. In addition, however, other compounds that have not yet been identified are responsible for a large part of the observed antioxidant capacity. Keywords: Euterpe oleracea; açaí; seed extract; TOSC assay; antioxidant; peroxyl radicals; peroxynitrite; hydroxyl radicals; procyanidins
ISSN:0021-8561
1520-5118
DOI:10.1021/jf058169p