F-actin, beta-tubulin, aldolase, and fructose-1,6-bisphosphatase in heteropteran ovarioles--I. Immunocytochemical investigations of whole-mounted ovarioles

The distribution of F-actin, beta-tubulin, aldolase, and fructose-1,6-bisphosphatase (FBPase) in ovarioles of four heteropteran species (Ilyocoris cimicoides, Coreus marginatus, Lygus pratensis, and Notostira elongata) was investigated biochemically and immunocytochemically. Aldolase was found to be...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue research 2005-08, Vol.321 (2), p.311-321
Main Authors: Dziewulska-Szwajkowska, D, Ogorzalek, A
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Cytoplasm - metabolism
Cytoplasm - ultrastructure
Female
Fructose-Bisphosphatase - metabolism
Fructose-Bisphosphate Aldolase - metabolism
Germ Cells - metabolism
Germ Cells - ultrastructure
Heteroptera - cytology
Heteroptera - metabolism
Immunohistochemistry
Ovarian Follicle - cytology
Ovarian Follicle - metabolism
Ovary - cytology
Ovary - metabolism
Reproduction - physiology
Stress Fibers - metabolism
Stress Fibers - ultrastructure
Tubulin - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The distribution of F-actin, beta-tubulin, aldolase, and fructose-1,6-bisphosphatase (FBPase) in ovarioles of four heteropteran species (Ilyocoris cimicoides, Coreus marginatus, Lygus pratensis, and Notostira elongata) was investigated biochemically and immunocytochemically. Aldolase was found to be uniformly distributed in the cytoplasm of trophocytes and follicular cells, with the highest concentration in prefollicular cells. Its concentration in follicular cells increased during differentiation and reached a peak in ovarian follicles at the stage of late choriogenesis. FBPase was observed in the cytoplasm (weak reaction) and on cell borders (strong reaction) of both germ line and somatic cells. No FBPase or aldolase signal was observed on the F-actin trophic core mesh or on stress fibers.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-005-1146-0