Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of t...

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Bibliographic Details
Published in:Virus research 2005-09, Vol.112 (1), p.38-41
Main Authors: Abe, Takumi, Miyake, Norifumi, Nishijima, Yasuyuki, Fujita, Ryousuke, Sahara, Ken, Asano, Shin-ichiro, Bando, Hisanori
Format: Article
Language:English
Subjects:
35S promoter
Animals
Autographa californica
Baculovirus
Base Sequence
CaMV
Cauliflower mosaic virus
Caulimovirus - genetics
Cells, Cultured
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Gene Expression Regulation, Viral
Luciferases - genetics
Luciferases - metabolism
Molecular Sequence Data
Nuclear polyhedrosis virus
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - physiology
Promoter Regions, Genetic - genetics
Promoter Regions, Genetic - physiology
Recombination, Genetic
Spodoptera - virology
Spodoptera frugiperda
Transcription, Genetic
Viral-induced RNA polymerase
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Summary:We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2005.03.019