Effect of Grain Source and Exogenous Phytase on Phosphorus Digestibility in Dairy Cows

• Published in: Journal of dairy science 2005-08, Vol.88 (8), p.2893-2902
• Main Authors: Kincaid, R. L, Garikipati, D. K, Nennich, T. D, Harrison, J. H
• Format: Article
• Language: English
• Subjects: 6-Phytase - administration & dosage; 6-Phytase - metabolism; Animal Nutritional Physiological Phenomena; Animal productions; Animals; Biological and medical sciences; Calcium - analysis; Calcium - blood; cattle; Cattle - physiology; Cereal and baking product industries; cultivars; Dairy cattle; Diet; Dietary Proteins - administration & dosage; Dietary Proteins - analysis; Digestion; Eating; Edible Grain; Fatty Acids, Volatile - analysis; Feces - chemistry; Female; Food industries; Fundamental and applied biological sciences. Psychology; Hordeum - chemistry; Hordeum vulgare; Hydrolysis; Intestinal Absorption; Lactation; Milk - chemistry; milk yield; phosphorus; Phosphorus - administration & dosage; Phosphorus - analysis; Phosphorus - pharmacokinetics; phytase; phytate; Phytic Acid - chemistry; Rumen - chemistry; Rumen - metabolism; rumen fermentation; Terrestrial animal productions; Vertebrates; Zea mays - chemistry
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(05)72970-2

Two experiments were conducted to determine P digestibility in lactating dairy cows fed corn or barley as grain sources. The first experiment utilized a replicated incomplete 5×4 Latin square design with 8 lactating Holstein cows fed diets containing either corn alone or corn in combination with one of 4 barley varieties that differed in chemical composition. Total tract digestibility of P ranged from 11 to 29% for diets containing the barley varieties and was approximately 35% for the corn diet. A second experiment compared P digestibility in cows fed diets containing corn or barley when exogenous phytase was added to the diets. Lactating Holstein cows (n=16) were arranged in 4 replications of a Latin square with 2 grains (barley or corn), fed separately or with added exogenous phytase (427 phytase units/kg of total mixed ration and 4 periods of 21 d. Phytate P comprised about 50% of the total P (0.46% P) in the total mixed ration. The concentration of serum inorganic P was higher in cows fed diets with exogenous phytase (5.8 vs. 6.5mg/dL in cows fed barley diets and 5.5 vs 6.0mg/dL in cows fed corn diets). Using acid detergent lignin as an internal marker, hydrolysis of phytate P was increased by the exogenous phytase, and total P digestibility tended to be increased. In contrast to Experiment 1, in Experiment 2 there was no effect of grain source on P digestibility and total fecal P. Dry matter intake and efficiency of milk production were not affected by exogenous phytase or grain type. Although phytase activity occurs in the rumen, physical properties of the diet and ruminal passage rates may prevent total hydrolysis of phytate in the rumen of lactating cows. Thus, exogenous dietary phytase might improve P digestibility in dairy cows in some dietary situations.