Characterization of β-Hydroxy-β-methylglutaryl Coenzyme A Reductase Inhibitor from Pueraria thunbergiana

• Published in: Journal of agricultural and food chemistry 2005-07, Vol.53 (15), p.5882-5888
• Main Authors: Kim, Hyoung-Jong, Lee, Dae-Hyoung, Hwang, Yoo-Yeon, Lee, Ki-Sung, Lee, Jong-Soo
• Format: Article
• Language: English
• Subjects: Animals; antilipemic agents; Biological and medical sciences; Cercopithecus aethiops; Cricetinae; daidzein; enzyme inhibition; enzyme inhibitors; Food industries; Fundamental and applied biological sciences. Psychology; Hydroxymethylglutaryl CoA Reductases - metabolism; hydroxymethylglutaryl-CoA lyase; Hydroxymethylglutaryl-CoA Reductase Inhibitors - isolation & purification; Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology; Hypolipidemic Agents - pharmacology; medicinal plants; Mesocricetus; plant extracts; Pueraria - chemistry; Pueraria montana var. lobata; Vero Cells
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0505978

This study describes the extraction and characterization of an inhibitor for β-hydroxy-β-methylglutaryl (HMG) coenzyme A (CoA) reductase from Pueraria thunbergiana. The maximum HMG-CoA reductase inhibitory activity (IC50 = 79 μg) was obtained when P. thunbergiana was extracted with 70% ethanol at 30 °C for 12 h. After purification of the HMG-CoA reductase inhibitor by means of systematic solvent extraction, silica gel column chromatography, and HPLC, an active fraction with an IC50 of 0.9 μg (4.25 μM) and a yield of 1.3% was obtained. The purified HMG-CoA reductase inhibitor was identified as daidzein (C15H10O4; molecular mass, 254 Da). Keywords: Pueraria thunbergiana; β-hydroxy-β-methylglutaryl coenzyme A reductase inhibitor