Nucleotide sequence and genomic organization of a newly isolated densovirus infecting Dendrolimus punctatus

• Published in: Journal of general virology 2005-08, Vol.86 (8), p.2169-2173
• Main Authors: Wang, Junping, Zhang, Jiamin, Jiang, Hong, Liu, Chuanfeng, Yi, Fuming, Hu, Yuanyang
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Bombyx mori; Capsid Proteins - genetics; Casphalia extranea densovirus; Dendrolimus punctatus; Densovirus; Densovirus - genetics; DNA Helicases - genetics; DNA, Viral; DNA-Binding Proteins - genetics; Fundamental and applied biological sciences. Psychology; Genome, Viral; Insect Viruses - genetics; Iteravirus; Larva - virology; Microbiology; Miscellaneous; Molecular Sequence Data; Moths - virology; Nucleic Acid Conformation; Open Reading Frames; Phospholipases A - genetics; Phospholipases A2; Phylogeny; Protein Structure, Tertiary - genetics; Trans-Activators - genetics; Viral Nonstructural Proteins - genetics; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/vir.0.80898-0

Laboratory of Insect Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China Correspondence Yuanyang Hu yyhu{at}whu.edu.cn The nucleotide sequence of a novel icosahedral DNA virus infecting Dendrolimus punctatus has been determined. The genome is 5039 nt long and includes inverted terminal repeats of 200 nt containing 131 nt long J-shaped terminal hairpins. The ‘plus' strand of the genome contains three large open reading frames (ORFs), the left and the mid-ORFs (within the left ORF) in the left-half encoding the non-structural proteins and the right ORF in the right-half encoding viral capsid proteins. NS1 protein contains conserved replication initiation and DNA-dependent ATPase/helicase domains. VP1 protein contains a conserved PGY and phospholipase A2 motifs and shows high identities with VPs of Casphalia extranea densovirus and Bombyx mori densovirus-1 belonging to the genus Iteravirus . Phylogenetic analysis also revealed that this virus is most closely related to Casphalia extranea densovirus and Bombyx mori densovirus-1. Consequently, this virus was considered as a new third member of the genus Iteravirus of the subfamily Densovirinae , and designated Dendrolimus punctatus densovirus. The GenBank/EMBL/DDBJ accession number of the nucleotide sequence reported in this paper is AY665654 .